Wellcome Centre for Mitochondrial Research, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle upon Tyne, UK.
Bioimaging Unit, Newcastle University, Faculty of Medical Sciences, Newcastle upon Tyne, UK.
Methods Mol Biol. 2023;2661:303-316. doi: 10.1007/978-1-0716-3171-3_17.
High-resolution imaging has enabled scientists to explore the mitochondrial network at remarkable resolution. This has been exploited to help increase our knowledge of how mitochondrial gene expression is compartmentalized in cultured cells. Here, we provide detailed methodology to simultaneously visualize up to four components including mtDNA-encoded transcripts, submitochondrial marker proteins, mitoribosomal subunits, or core members of the translational apparatus using STED super-resolution nanoscopy.
高分辨率成像使科学家能够以惊人的分辨率探索线粒体网络。这一技术被用于帮助增加我们对线粒体基因表达在培养细胞中如何分隔的认识。在这里，我们提供了详细的方法学，使用 STED 超分辨率纳米显微镜同时可视化多达四个组件，包括线粒体 DNA 编码的转录本、线粒体亚细胞器标记蛋白、线粒体核糖体亚单位或翻译装置的核心成员。
