Department of Diagnosis and Surgery-Periodontics Division, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Nucleus of Study and Research in Periodontology and Implantology (NEPPI), School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
PLoS One. 2023 May 11;18(5):e0284497. doi: 10.1371/journal.pone.0284497. eCollection 2023.
The aim of this study was to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats.
Thirty 3-month-old male rats were used. EP was induced around the mandibular first molars (right and left). Seven days after induction, the treatments with either HD (n = 30) or UDS (n = 30) were randomly performed in each molar. Euthanasia were performed at 7, 15, and 30 days after treatment. Histometric (percentage of bone in the furcation [PBF]), histopathological, and immunohistochemical (for detection of tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]). Parametric data (PBF and TRAP) was analyzed by One-way ANOVA followed by Tukey's post-test. OCN was analyzed by Kruskal-Wallis followed by Student-Newman-Keuls post-test. The level of significance was 5%.
Group HD presented higher PBF and lower TRAP-immunolabeling at 30 days as compared with UDS in the same period (p≤0.05). Group HD presented higher OCN immunolabeling at 30 days as compared with 7 and 15 days (p≤0.05). Persistent and exacerbated inflammatory process was observed in some specimens from group UDS at 30 days, as well as the bone trabeculae presented irregular contour, surrounded by many active osteoclasts.
Nonsurgical periodontal therapy with HD resulted in higher PBF and lower expression of TRAP as compared with UDS. Also, HD increased the expression of OCN over time.
本研究旨在评估手动清创术（HD）与超声牙科洁牙器（UDS）治疗大鼠实验性牙周炎（EP）的疗效。
使用 30 只 3 月龄雄性大鼠。在右下颌第一磨牙周围诱导 EP。诱导后 7 天，随机对每颗磨牙进行 HD（n=30）或 UDS（n=30）治疗。治疗后 7、15 和 30 天进行安乐死。进行组织计量学（分叉处骨百分比 [PBF]）、组织病理学和免疫组织化学（检测抗酒石酸酸性磷酸酶 [TRAP]和骨钙素 [OCN]）检查。参数数据（PBF 和 TRAP）采用单因素方差分析，然后进行 Tukey 事后检验。OCN 采用 Kruskal-Wallis 检验，然后进行 Student-Newman-Keuls 事后检验。显著性水平为 5%。
与同期 UDS 相比，HD 组在 30 天时 PBF 更高，TRAP 免疫标记物更低（p≤0.05）。与 7 天和 15 天相比，HD 组在 30 天时 OCN 免疫标记物更高（p≤0.05）。在 UDS 组的一些标本中，在 30 天时仍观察到持续和加重的炎症过程，骨小梁轮廓不规则，周围有许多活跃的破骨细胞。
与 UDS 相比，HD 进行的非手术牙周治疗导致 PBF 更高，TRAP 表达更低。此外，HD 随着时间的推移增加了 OCN 的表达。
