巴西人群接种灭活 SARS-CoV-2 后抗体应答相关的代谢适应性研究。

Metabolic Adaptations Correlated with Antibody Response after Immunization with Inactivated SARS-CoV-2 in Brazilian Subjects.

机构信息

Histocompatibility and Cryopreservation Laboratory, IBRAG, Rio de Janeiro State University, CEP 20950-003 Rio de Janeiro, Rio de Janeiro, Brazil.

Department of Genetics, IBRAG, Rio de Janeiro State University, CEP 20550-013 Rio de Janeiro, Rio de Janeiro, Brazil.

出版信息

J Proteome Res. 2023 Jun 2;22(6):1908-1922. doi: 10.1021/acs.jproteome.3c00014. Epub 2023 May 11.

DOI:10.1021/acs.jproteome.3c00014

PMID:37167433

Abstract

The adsorbed vaccine SARS-CoV-2 (inactivated) produced by Sinovac (SV) was the first vaccine against COVID-19 to be used in Brazil. To understand the metabolic effects of SV in Brazilian subjects, NMR-based metabolomics was used, and the immune response was studied in Brazilian subjects. Forty adults without (group, = 23) and with previous COVID-19 infection (group, = 17) were followed-up for 90 days postcompletion of the vaccine regimen. After 90 days, our results showed that subjects had increased levels of lipoproteins, lipids, and -acetylation of glycoproteins (NAG) as well as decreased levels of amino acids, lactate, citrate, and 3-hydroxypropionate. NAG and threonine were the highest correlated metabolites with N and S proteins, and neutralizing Ab levels. This study sheds light on the immunometabolism associated with the use of SV in Brazilian subjects from Rio de Janeiro and identifies potential metabolic markers associated with the immune status.

摘要

由科兴（SV）生产的吸附疫苗 SARS-CoV-2（灭活）是在巴西使用的第一种 COVID-19 疫苗。为了了解 SV 在巴西受试者中的代谢效应，我们使用了基于 NMR 的代谢组学，并在巴西受试者中研究了免疫反应。40 名未感染 COVID-19 的成年人（组，n=23）和既往感染 COVID-19 的成年人（组，n=17）在完成疫苗接种方案后随访 90 天。90 天后，我们的结果表明，受试者的脂蛋白、脂质和糖蛋白的β-乙酰化（NAG）水平升高，而氨基酸、乳酸盐、柠檬酸盐和 3-羟丙酸的水平降低。NAG 和苏氨酸是与 N 和 S 蛋白以及中和抗体水平相关性最高的代谢物。这项研究揭示了与里约热内卢巴西受试者使用 SV 相关的免疫代谢，并确定了与免疫状态相关的潜在代谢标志物。