Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Henan Key Laboratory of Tumor Pathology, Zhengzhou University, Zhengzhou, China.
Pathol Oncol Res. 2023 Apr 24;29:1611081. doi: 10.3389/pore.2023.1611081. eCollection 2023.
Despite significant advances in the diagnosis and treatment of esophageal squamous cell carcinoma (ESCC), esophageal cancer is still a heavy social and medical burden due to its high incidence. Uncontrolled division and proliferation is one of the characteristics of tumor cells, which will promote rapid tumor growth and metastasis. Early mitotic inhibitor 1 (Emi1), ubiquitin-conjugating enzyme 10 (UBCH10) and CyclinB1 are important proteins involved in the regulation of cell cycle. In this study, the expression of Emi1, UBCH10 and CyclinB1 in ESCC tissues and adjacent normal tissues will be analyzed by immunohistochemistry and hybridization techniques, and their relationship with tumor proliferation and apoptosis will be analyzed. The results showed that Emi1, UBCH10 and CyclinB1 genes and proteins were highly expressed in tumor tissues, which were correlated with tumor grade, lymph node metastasis and pathological stage, and positively correlated with tumor proliferation. Emi1, UBCH10 and CyclinB1 are also positively correlated. It is speculated that Emi1, UBCH10 and CyclinB1 genes synergically promote tumor proliferation and inhibit apoptosis, which may be potential diagnostic and therapeutic targets for ESCC.
尽管在食管鳞状细胞癌 (ESCC) 的诊断和治疗方面取得了重大进展，但由于其高发病率，食管癌仍然是一个沉重的社会和医疗负担。不受控制的分裂和增殖是肿瘤细胞的特征之一，这将促进肿瘤的快速生长和转移。早期有丝分裂抑制剂 1 (Emi1)、泛素连接酶 10 (UBCH10) 和细胞周期蛋白 B1 是参与细胞周期调节的重要蛋白质。在这项研究中，将通过免疫组织化学和杂交技术分析 ESCC 组织和相邻正常组织中 Emi1、UBCH10 和 CyclinB1 的表达，并分析它们与肿瘤增殖和凋亡的关系。结果表明，Emi1、UBCH10 和 CyclinB1 基因和蛋白在肿瘤组织中高表达，与肿瘤分级、淋巴结转移和病理分期相关，与肿瘤增殖呈正相关。Emi1、UBCH10 和 CyclinB1 也呈正相关。推测 Emi1、UBCH10 和 CyclinB1 基因协同促进肿瘤增殖并抑制凋亡，这可能是 ESCC 的潜在诊断和治疗靶点。
