Zhu Zaifu, Zhang Wei, Huo Shaohu, Huang Tiantuo, Cao Xi, Zhang Ying
Department of Pediatrics, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Department of Rehabilitation Medicine, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Front Mol Biosci. 2024 May 2;11:1365655. doi: 10.3389/fmolb.2024.1365655. eCollection 2024.
TUBB can encode a beta-tubulin protein. At present, the role of TUBB has not been ascertained in cancers. Hence, the importance of further systematic pan-cancer analyses is stressed to explore its value in the diagnosis, prognosis, and immune function of cancers.
By collecting and handling integrative data from the TCGA, Firehose, UCSC Xena, cBioPortal, GEO, CPTAC, TIMER2.0, TISCH, CellMiner, GDSC, and CTRP databases, we explored the potential diagnostic and prognostic roles of TUBB in pan-cancers from multiple angles. Moreover, the GSEA analysis was conducted to excavate the biological functions of TUBB in pan-cancers. In addition, survival profiles were described, and the differential expressions of TUBB in different molecular subtypes were discussed. Also, we utilized the cMAP function to search drugs or micro-molecules that have an impact on TUBB expressions.
Based on the TCGA data, we found that TUBB was differentially expressed in a variety of tumors and showed an early-diagnostic value. Mutations, somatic copy number alterations, and DNA methylation would lead to its abnormal expression. TUBB expressions had relations with many clinical features. What's more, TUBB expressions were validated to be related to many metabolism-related, metastasis-related, and immune-related pathways. High TUBB expressions were proved to have a great impact on the prognosis of various types of cancers and would affect the sensitivity of some drugs. We also demonstrated that the expression of TUBB was significantly correlated to immunoregulator molecules and biomarkers of lymphocyte subpopulation infiltration.
TUBB and its regulatory genes were systemically analyzed in this study, showing that TUBB had satisfying performances in disease diagnosing and prognosis predicting of multiple cancers. It could remodel the tumor microenvironment and play an integral role in guiding cancer therapies and forecasting responses to chemotherapy.
TUBB可编码一种β-微管蛋白。目前，TUBB在癌症中的作用尚未明确。因此，强调进一步进行系统的泛癌分析以探索其在癌症诊断、预后及免疫功能方面价值的重要性。
通过收集和处理来自TCGA、Firehose、UCSC Xena、cBioPortal、GEO、CPTAC、TIMER2.0、TISCH、CellMiner、GDSC和CTRP数据库的整合数据，我们从多个角度探索了TUBB在泛癌中的潜在诊断和预后作用。此外，进行基因集富集分析（GSEA）以挖掘TUBB在泛癌中的生物学功能。另外，描述了生存曲线，并讨论了TUBB在不同分子亚型中的差异表达。我们还利用cMAP功能搜索对TUBB表达有影响的药物或小分子。
基于TCGA数据，我们发现TUBB在多种肿瘤中存在差异表达，并具有早期诊断价值。突变、体细胞拷贝数改变和DNA甲基化会导致其异常表达。TUBB表达与许多临床特征相关。此外，TUBB表达被证实与许多代谢相关、转移相关和免疫相关途径有关。高TUBB表达被证明对各种癌症的预后有很大影响，并会影响某些药物的敏感性。我们还证明TUBB的表达与免疫调节分子和淋巴细胞亚群浸润的生物标志物显著相关。
本研究对TUBB及其调控基因进行了系统分析，表明TUBB在多种癌症的疾病诊断和预后预测方面表现良好。它可以重塑肿瘤微环境，并在指导癌症治疗和预测化疗反应中发挥不可或缺的作用。
