State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Proteomics. 2023 Aug;23(15):e2200437. doi: 10.1002/pmic.202200437. Epub 2023 May 12.
Lactate is closely related to various cellular processes, such as angiogenesis, responses to hypoxia, and macrophage polarization, while regulating natural immune signaling pathways and promoting neurogenesis and cognitive function. Lysine lactylation (Kla) is a novel posttranslational modification, the examination of which may lead to new understanding of the nonmetabolic functions of lactate and the various physiological and pathological processes in which lactate is involved, such as infection, tumorigenesis and tumor development. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), researchers have identified lactylation in human gastric cancer cells and some other species, but no research on lactylation in human lungs has been reported. In this study, we performed global profiling of lactylation in human lungs under normal physiological conditions, and 724 Kla sites in 451 proteins were identified. After comparing the identified proteins with those reported in human lactylation datasets, 141 proteins that undergo lactylation were identified for the first time in this study. Our work expands the database on human lactylation and helps advance the study on lactylation function and regulation under physiological and pathological conditions.
乳酸与多种细胞过程密切相关，如血管生成、对缺氧的反应和巨噬细胞极化，同时调节天然免疫信号通路，促进神经发生和认知功能。赖氨酸乳酸化（Kla）是一种新的翻译后修饰，对其的研究可能会导致对乳酸非代谢功能的新认识，以及乳酸参与的各种生理和病理过程，如感染、肿瘤发生和肿瘤发展。研究人员使用液相色谱-串联质谱（LC-MS/MS）在人胃癌细胞和其他一些物种中鉴定出了乳酸化，但尚未有关于人肺乳酸化的研究报告。在这项研究中，我们在正常生理条件下对人肺中的乳酸化进行了全局分析，鉴定出 451 种蛋白质中的 724 个 Kla 位点。将鉴定出的蛋白质与在人乳酸化数据集报告的蛋白质进行比较后，首次在本研究中鉴定出 141 种发生乳酸化的蛋白质。我们的工作扩展了人类乳酸化数据库，并有助于推进在生理和病理条件下对乳酸化功能和调控的研究。
