Mingorance Álvarez Esther, Villar Rodríguez Julia, López Ripado Olga, Mayordomo Raquel
Department of Physiology, University Centre of Mérida, University of Extremadura, 06800 Mérida, Badajoz, Spain.
Department of Nursing, Physiotherapy and Occupational Therapy, Faculty of Health Sciences, University of Castilla la Mancha, 45600 Talavera de la Reina, Toledo, Spain.
J Fungi (Basel). 2024 Sep 27;10(10):675. doi: 10.3390/jof10100675.
Onychomycosis is a common fungal infection that affects the nails and accounts for approximately 50% of all nail diseases. The main pathogens involved include dermatophytes, such as , members of the complex, and emerging pathogens in this infection, and . Tea tree ( Cheel) essential oil (EO) has been proposed as a promising natural alternative to traditional treatments due to its antimicrobial properties. Among its more than 100 compounds, terpinen-4-ol is one of the main contributors to the antifungal action of this EO. To determine the antifungal activity of tea tree EO against dermatophytes, we designed an study using EUCAST-AFST protocols to obtain the values of MIC (minimum inhibitory concentration) and MFC (minimum fungicidal concentration) of several commercial Cheel EOs against three species of dermatophytes isolated from clinical samples with suspected toenail onychomycosis. The results showed that the microorganism most sensitive to the action of the EO was , which had an MIC value more than 13 times lower than the value obtained for (0.4% /), the most resistant isolate. No differences in antifungal activity were observed by the analysed EOs or between the MIC and MFC values. These results suggest that tea tree EO is a viable option for the alternative treatment of onychomycosis, although clinical studies are needed to confirm the long-term antifungal activity, safety and efficacy of the oils studied in a clinical context.
甲癣是一种常见的真菌感染，会影响指甲，约占所有指甲疾病的50%。主要涉及的病原体包括皮肤癣菌，如 复合体的成员，以及这种感染中的新兴病原体 和 。茶树（Melaleuca alternifolia Cheel）精油（EO）因其抗菌特性，已被提议作为传统治疗方法的一种有前景的天然替代方案。在其100多种化合物中，萜品-4-醇是这种精油抗真菌作用的主要贡献者之一。为了确定茶树精油对皮肤癣菌的抗真菌活性，我们设计了一项研究，使用EUCAST-AFST方案来获取几种市售互叶白千层精油对从疑似趾甲甲癣临床样本中分离出的三种皮肤癣菌的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）值。结果表明，对精油作用最敏感的微生物是 ，其MIC值比最耐药的分离株 （0.4% /）的MIC值低13倍以上。分析的精油之间或MIC和MFC值之间未观察到抗真菌活性的差异。这些结果表明，茶树精油是甲癣替代治疗的一个可行选择，尽管需要进行临床研究来证实所研究的精油在临床环境中的长期抗真菌活性、安全性和有效性。
