RING 指蛋白 NbRFP1 参与调控燕麦丛矮病毒 RepA 诱导的宿主过敏反应。

The RING-Finger Protein NbRFP1 Contributes to Regulating the Host Hypersensitive Response Induced by Oat Dwarf Virus RepA.

机构信息

Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

College of Life Science, Huzhou University, Huzhou 313000, China.

出版信息

Int J Mol Sci. 2023 Apr 22;24(9):7697. doi: 10.3390/ijms24097697.

DOI:10.3390/ijms24097697

PMID:37175403

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10178201/

Abstract

Our previous study identified that the RepA protein encoded by the oat dwarf virus (ODV) was responsible for inducing a strong hypersensitive response (HR) during the virus infection in non-host tobacco plants. However, little was known about the molecular mechanism of the RepA-elicited HR. Here, a RING-finger protein, which is described as NbRFP1 and is mainly located in the cytoplasm and nucleus in cells, was confirmed to interact with RepA. In addition, the accumulation level of in leaves was enhanced by either ODV infection or by only RepA expression. The knockdown of by a TRV-mediated virus-induced gene silencing markedly delayed the ODV or RepA-elicited HR. By contrast, the overexpression of in conferred enhanced resistance to ODV infection and promoted RepA-induced HR. Further mutation analysis showed that a RING-finger domain located in NbRFP1 plays important roles in modulating RepA-induced HR, as well as in mediating the interaction between NbRFP1 and RepA.

摘要

我们之前的研究表明， oat dwarf virus (ODV) 编码的 RepA 蛋白在非宿主烟草植物的病毒感染过程中诱导强烈的过敏反应 (HR)。然而，关于 RepA 诱导的 HR 的分子机制知之甚少。在这里，一种 RING 指蛋白，被描述为 NbRFP1，主要位于细胞质和细胞核中，被证实与 RepA 相互作用。此外，在叶片中，NbRFP1 的积累水平通过 ODV 感染或仅表达 RepA 而增强。通过 TRV 介导的病毒诱导基因沉默技术敲低会明显延迟 ODV 或 RepA 诱导的 HR。相比之下，在中过表达赋予对 ODV 感染的增强抗性，并促进 RepA 诱导的 HR。进一步的突变分析表明，位于 NbRFP1 中的一个 RING 指结构域在调节 RepA 诱导的 HR 以及介导 NbRFP1 和 RepA 之间的相互作用方面发挥重要作用。