Department of Physiology, Faculty of Medicine, Plaza Ramón y Cajal, s/n, Universidad Complutense, 28040 Madrid, Spain.
Scientific Department, Natac Biotech, 28923 Madrid, Spain.
Int J Mol Sci. 2023 Apr 28;24(9):7977. doi: 10.3390/ijms24097977.
Olive products contain high levels of monounsaturated fatty acids as well as other minor components such as triterpenic alcohols and other pentacyclic triterpenes, which together form the main triterpenes of virgin olive oil. Olive fruits and leaves contain significant amounts of hydrophilic and lipophilic bioactives including flavones, phenolic acids and phenolic alcohols, amongst others. Several studies have shown the benefits of these substances on the cardiovascular system. Regardless, little is known about the specific combination of bioactive compounds in cardiovascular health. Thus, we aimed to test the combination of a triterpenes (TT70) and a polyphenols (HT60) olive oil bioactive extract in H9c2 cells under stress conditions: LPS and HO stimulation. To evaluate the effectiveness of the combination, we measured cell viability, superoxide production and protein expression of caspase 3, eNOS, peNOS, TNF-α and Il-6. Overall, cells stimulated with LPS or HO and co-incubated with the combination of triterpenes and polyphenols had increased cell survival, lower levels of superoxide anion, lower protein expression of eNOS and higher expression of peNOS, increased protein expression of SOD-1 and lower protein expression of TNF-α and Il-6. The specific combination of HT60+TT70 is of great interest for further study as a possible treatment for cardiovascular damage.
橄榄油产品含有高水平的单不饱和脂肪酸以及其他少量成分，如三萜醇和其他五环三萜，它们共同构成了特级初榨橄榄油的主要三萜。橄榄果和叶含有大量亲水性和疏水性生物活性物质，包括类黄酮、酚酸和酚醇等。有几项研究表明这些物质对心血管系统有益。然而，对于心血管健康中生物活性化合物的特定组合知之甚少。因此，我们旨在测试三萜（TT70）和多酚（HT60）橄榄油生物活性提取物在应激条件下（LPS 和 HO 刺激）在 H9c2 细胞中的组合。为了评估组合的效果，我们测量了细胞活力、超氧化物产生以及 caspase 3、eNOS、peNOS、TNF-α 和 Il-6 的蛋白表达。总的来说，用 LPS 或 HO 刺激并与三萜和多酚的组合共同孵育的细胞具有更高的细胞存活率、更低的超氧阴离子水平、更低的 eNOS 蛋白表达和更高的 peNOS 表达、更高的 SOD-1 蛋白表达以及更低的 TNF-α 和 Il-6 蛋白表达。HT60+TT70 的特定组合非常有兴趣进一步研究作为治疗心血管损伤的可能方法。
