Fierascu Ioana Catalina, Fierascu Irina, Baroi Anda Maria, Ungureanu Camelia, Spinu Simona, Avramescu Sorin Marius, Somoghi Raluca, Fierascu Radu Claudiu, Dinu-Parvu Cristina Elena
Faculty of Pharmacy, University of Medicine and Pharmacy "Carol Davila", 37 Dionisie Lupu Str., 030167 Bucharest, Romania.
National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM Bucharest, 202 Spl. Independentei, 060021 Bucharest, Romania.
Materials (Basel). 2023 Apr 29;16(9):3472. doi: 10.3390/ma16093472.
The present work describes, for the first time in the literature, the phytosynthesis of silver nanoparticles using L. extracts. The influence of the extraction method (classical temperature extraction and microwave extraction), as well as of the extract concentration on the characteristics of the nanoparticles, was studied using analytical methods, such as UV-Vis spectrometry, X-ray diffraction, dynamic light scattering, and transmission electron microscopy. Experimental data suggest that use of lower extract concentration leads to smaller dimensions nanoparticles, the same effect using the extract obtained by microwave-assisted extraction. The smallest recorded crystallite sizes (by X-ray diffraction) were under 3 nm. The antioxidant properties (determined by the DPPH assay) and the antimicrobial potential (determined against Gram-negative and Gram-positive strains) are enhanced by the phytosynthesis process (as demonstrated by the comparison of the nanoparticles' properties with the parent extracts). The present work could also represent an important step in obtaining nanoparticles with enhanced properties and controlled morphologies, but also offers information on the phytosynthesis of metallic nanoparticles using low extract concentrations.
本研究首次在文献中描述了利用[植物名称]提取物进行银纳米颗粒的植物合成。使用紫外可见光谱、X射线衍射、动态光散射和透射电子显微镜等分析方法，研究了提取方法（经典温度提取和微波提取）以及提取物浓度对纳米颗粒特性的影响。实验数据表明，使用较低的提取物浓度会导致纳米颗粒尺寸更小，通过微波辅助提取获得的提取物也有相同效果。记录到的最小微晶尺寸（通过X射线衍射）小于3纳米。植物合成过程增强了抗氧化性能（通过DPPH测定法确定）和抗菌潜力（针对革兰氏阴性和革兰氏阳性菌株确定）（通过将纳米颗粒的特性与母体提取物进行比较证明）。本研究还可能是获得具有增强性能和可控形态的纳米颗粒的重要一步，同时也提供了关于使用低提取物浓度进行金属纳米颗粒植物合成的信息。
