Díaz-Puertas Rocío, Rodríguez-Cañas Enrique, Bello-Perez Melissa, Fernández-Oliver Marta, Mallavia Ricardo, Falco Alberto
Institute of Research, Development and Innovation in Healthcare Biotechnology of Elche (IDiBE), Miguel Hernández University (UMH), 03202 Elche, Spain.
Centro Nacional de Biotecnología (CNB-CSIC), Departamento de Biología Molecular y Celular, Campus de la Universidad Autónoma de Madrid, c/Darwin 3, 20849 Madrid, Spain.
Nanomaterials (Basel). 2023 Apr 25;13(9):1467. doi: 10.3390/nano13091467.
The use of diverse Ag-based nanoparticulated forms has shown promising results in controlling viral propagation. In this study, a commercial nanomaterial consisting of ceramic-coated silver nanoparticles (AgNPs) was incorporated into thermoplastic polyurethane (TPU) plates using an industrial protocol, and the surface composition, ion-release dynamics and viricidal properties were studied. The surface characterization by FESEM-EDX revealed that the molar composition of the ceramic material was 5.5 P:3.3 Mg:Al and facilitated the identification of the embedded AgNPs (54.4 ± 24.9 nm). As determined by ICPMS, the release rates from the AgNP-TPU into aqueous solvents were 4 ppm/h for Ag and Al, and 28.4 ppm/h for Mg ions. Regarding the biological assays, the AgNP-TPU material did not induce significant cytotoxicity in the cell lines employed. Its viricidal activity was characterized, based on ISO 21702:2019, using the Spring viraemia of carp virus (SVCV), and then tested against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The results demonstrated that AgNP-TPU materials exhibited significant (75%) and direct antiviral activity against SVCV virions in a time- and temperature-dependent manner. Similar inhibition levels were found against SARS-CoV-2. These findings show the potential of AgNP-TPU-based materials as a supporting strategy to control viral spread.
使用多种基于银的纳米颗粒形式在控制病毒传播方面已显示出有前景的结果。在本研究中，一种由陶瓷包覆银纳米颗粒（AgNPs）组成的商业纳米材料按照工业规程被掺入热塑性聚氨酯（TPU）板中，并对其表面组成、离子释放动力学和杀病毒特性进行了研究。通过场发射扫描电子显微镜-能谱仪（FESEM-EDX）进行的表面表征显示，陶瓷材料的摩尔组成为5.5磷：3.3镁：铝，这有助于识别嵌入的AgNPs（54.4±24.9纳米）。通过电感耦合等离子体质谱法（ICPMS）测定，AgNP-TPU在水性溶剂中的释放速率为：银和铝4 ppm/小时，镁离子28.4 ppm/小时。关于生物学测定，AgNP-TPU材料在所使用的细胞系中未诱导出显著的细胞毒性。根据ISO 21702:2019，使用鲤春病毒血症病毒（SVCV）对其杀病毒活性进行了表征，然后针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进行了测试。结果表明，AgNP-TPU材料对SVCV病毒粒子表现出显著的（75%）且直接的抗病毒活性，其活性呈时间和温度依赖性。对SARS-CoV-2也发现了类似的抑制水平。这些发现表明基于AgNP-TPU的材料作为控制病毒传播的辅助策略具有潜力。
