Feng Danni, Zhang Renyin, Zhang Mengting, Fang Ashe, Shi Feng
College of Life Science, Shihezi University, Shihezi 832003, China.
Nanomaterials (Basel). 2022 Jul 30;12(15):2636. doi: 10.3390/nano12152636.
In the present study, the biosynthesis of silver nanoparticles (AgNPs) and their antibacterial activity against gram-positive and gram-negative bacteria were investigated. Glycyrrhizin (GL) was used as a reducing agent and stabilizer to rapidly prepare the AgNPs. The distinctive absorption peak at 419 nm confirmed the formation of GL-reduced AgNPs. The TEM and particle size analysis shows that the prepared GL-reduced AgNPs were mostly circular with good dispersion and a relatively uniform particle size of 35 nm on average. Fourier transform infrared spectroscopy analysis was performed to identify the possible biomolecules in the capping and active stabilization of the GL-reduced AgNPs. The antibacterial activity of the GL-reduced AgNPs was analyzed with the Oxford cup diffusion method and filter paper diffusion method. The experimental results show that these properties endowed the GL-reduced AgNPs with high antibacterial activity against and and lay a foundation for the use of colloidal silver in antibacterial applications. The GL-reduced AgNPs also had stronger antibacterial activity than sodium citrate-reduced AgNPs, which indicates the advantages of GL-reduced AgNPs compared with sodium citrate-reduced AgNPs in inducing bacteriostasis. The cytotoxicity of GL-reduced AgNPs on human kidney epithelial 293A (HEK293) cells was evaluated via the MTT assay. The results show that GL-reduced AgNPs had lower toxicity to HEK293 cells than sodium citrate-AgNPs, which indicates that the as-prepared GL-reduced AgNPs are environmentally friendly.
在本研究中，对银纳米颗粒（AgNPs）的生物合成及其对革兰氏阳性菌和革兰氏阴性菌的抗菌活性进行了研究。甘草酸（GL）被用作还原剂和稳定剂以快速制备AgNPs。在419nm处的独特吸收峰证实了GL还原的AgNPs的形成。透射电子显微镜（TEM）和粒度分析表明，所制备的GL还原的AgNPs大多为圆形，分散性良好，平均粒径相对均匀，为35nm。进行了傅里叶变换红外光谱分析，以确定在GL还原的AgNPs的封端和活性稳定中可能存在的生物分子。采用牛津杯扩散法和滤纸扩散法分析了GL还原的AgNPs的抗菌活性。实验结果表明，这些特性赋予了GL还原的AgNPs对[具体革兰氏阳性菌]和[具体革兰氏阴性菌]的高抗菌活性，并为胶体银在抗菌应用中的使用奠定了基础。GL还原的AgNPs也比柠檬酸钠还原的AgNPs具有更强的抗菌活性，这表明GL还原的AgNPs在诱导抑菌方面比柠檬酸钠还原的AgNPs具有优势。通过MTT法评估了GL还原的AgNPs对人肾上皮293A（HEK293）细胞的细胞毒性。结果表明，GL还原的AgNPs对HEK293细胞的毒性低于柠檬酸钠-AgNPs，这表明所制备的GL还原的AgNPs是环境友好的。
