Teimoorian Mehran, Mirzaie Maysam, Tashakkorian Hamed, Gholinia Hemmat, Alaghemand Homayoon, Pournajaf Abazar, Ghorbanipour Reza
Student Research Committee, Faculty of Dentistry, Babol University of Medical Sciences, Babol, I.R.Iran.
Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran.
Dent Res J (Isfahan). 2023 Mar 28;20:37. eCollection 2023.
Polymethyl methacrylate resin is widely used in orthodontic treatments. Graphene oxide (GO) has reactive functional groups on its surface that facilitate binding to various materials such as polymers, biomolecules, DNA, and proteins. This study aimed to investigate the impact of adding functionalized GO nanosheets on the physical, mechanical, cytotoxicity, and anti-biofilm properties of acrylic resin.
In this experimental study, fifty samples (for each test) were divided into groups of 10, in the form of acrylic resin discs with concentrations of 0, 0.25, 0.5, 1, and 2 weight percentage (wt%) of functionalized GO nanosheets and also the control group. Samples were evaluated in terms of physical properties (surface hardness, surface roughness, compressive strength, fracture toughness, and flexural strength), anti-biofilm properties (On four groups of micro-organisms, including , , , and ), and cytotoxicity. Data were analyzed using SPSS software version 22, descriptive statistics, one-way analysis of variance test, and Tukey test. The significance level was considered < 0.05.
No significant difference was observed between the different groups with weight percentages of 0.25, 0.5, 1, and 2% nano GO (nGO) and the control group (without nGO) in terms of surface roughness and toughness. However, compressive strength, three-point flexural strength, and surface hardness showed significant differences between the groups. Furthermore, the degree of cytotoxicity increased by increasing the weight percentage of nano-GO.
The addition of functionalized nGO in appropriate concentrations to polymethyl methacrylate can improve the anti-bacterial and anti-fungal biofilm properties without changing or increasing their physical and mechanical properties.
聚甲基丙烯酸甲酯树脂广泛应用于正畸治疗。氧化石墨烯（GO）表面具有反应性官能团，便于与聚合物、生物分子、DNA和蛋白质等各种材料结合。本研究旨在探讨添加功能化GO纳米片对丙烯酸树脂的物理、机械、细胞毒性和抗生物膜性能的影响。
在本实验研究中，五十个样品（每个测试）以含0、0.25、0.5、1和2重量百分比（wt%）功能化GO纳米片的丙烯酸树脂圆盘形式分为每组10个的组，还有对照组。对样品进行物理性能（表面硬度、表面粗糙度、抗压强度、断裂韧性和弯曲强度）、抗生物膜性能（对四组微生物，包括、、和）和细胞毒性评估。使用SPSS软件22版进行数据分析，包括描述性统计、单因素方差分析和Tukey检验。显著性水平设定为<0.05。
在表面粗糙度和韧性方面，重量百分比为0.25%、0.5%、1%和2%的纳米GO（nGO）组与对照组（无nGO）之间未观察到显著差异。然而，抗压强度、三点弯曲强度和表面硬度在各组之间存在显著差异。此外，细胞毒性程度随纳米GO重量百分比的增加而增加。
向聚甲基丙烯酸甲酯中添加适当浓度的功能化nGO可改善其抗细菌和抗真菌生物膜性能，而不会改变或增加其物理和机械性能。
