Yu Lu, Xie Hairong, Wang Linping, Cheng Min, Liu Jie, Xu Jiamei, Wei Zhigang, Ye Xin, Xie Qi, Liang Jing
Department of Oncology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Shandong Lung Cancer Institute, Jinan, China.
Department of Oncology, Feicheng People's Hospital, Feicheng, China.
Front Oncol. 2023 Apr 27;13:1174713. doi: 10.3389/fonc.2023.1174713. eCollection 2023.
Thermal ablation is the primary procedure for the local treatment of lung metastases. It is known that radiotherapy and cryoablation can stimulate an abscopal effect, while the occurrence of abscopal effect induced by microwave ablation is less; the cellular and molecular mechanisms involved in the abscopal effect after microwave ablation should be further elucidated.
CT26 tumor-bearing Balb/c mice were treated with microwave ablation with several combinations of ablation power and time duration. The growth of primary or abscopal tumors and the survival of mice were both monitored; moreover, immune profiles in abscopal tumors, spleens, and lymph nodes were examined by flow cytometry.
Microwave ablation suppressed tumor growth in both primary and abscopal tumors. Both local and systemic T-cell responses were induced by microwave ablation. Furthermore, the mice exhibiting significant abscopal effect after microwave ablation markedly elevated Th1 cell proportion both in the abscopal tumors and spleens.
Microwave ablation at 3 w-3 min not only suppressed tumor growth in the primary tumors but also stimulated an abscopal effect in the CT26-bearing mice the improvement of systemic and intratumoral antitumor immunity.
热消融是肺转移瘤局部治疗的主要方法。已知放疗和冷冻消融可激发远隔效应，而微波消融诱导远隔效应的情况较少；微波消融后远隔效应所涉及的细胞和分子机制有待进一步阐明。
对荷CT26肿瘤的Balb/c小鼠采用多种消融功率和持续时间组合进行微波消融治疗。监测原发肿瘤或远隔肿瘤的生长及小鼠的生存情况；此外，通过流式细胞术检测远隔肿瘤、脾脏和淋巴结中的免疫谱。
微波消融抑制了原发肿瘤和远隔肿瘤的生长。微波消融诱导了局部和全身的T细胞反应。此外，微波消融后表现出明显远隔效应的小鼠，其远隔肿瘤和脾脏中的Th1细胞比例均显著升高。
3W-3min的微波消融不仅抑制了原发肿瘤的生长，还在荷CT26小鼠中激发了远隔效应——全身和肿瘤内抗肿瘤免疫力的提高。
