State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
Mikrochim Acta. 2023 May 15;190(6):223. doi: 10.1007/s00604-023-05793-0.
This study aimed to design a sandwich electrochemiluminescence (ECL) immunosensor with double co-reaction accelerators for sensitively detecting squamous cell carcinoma antigen (SCCA). First, silver orthophosphate (AgPO) nanoparticles were modified on the surface of EuPO nanowires to improve their poor dispersibility/solubility. At the same time, EuPO was used as a co-reaction accelerator to catalyze SO to produce more intermediates (SO), significantly enhancing the ECL signal of AgPO. Ag nanoparticles (AgNP) modified on AgPO@EuPO composite nanomaterials were used not only as linkers of luminescence groups and biomarkers but also as a co-reaction accelerator to effectively enhance ECL signal. The designed ECL immunosensor displayed several advantages, including good stability and reproducibility. Under the optimal conditions, its linear range in detecting SCCA was 0.0001-50 ng·mL, the detection limit was 25 fg·mL (S/N = 3), the recovery was 96.6-100.4%, and the relative standard deviation was less than 4.8%. It was successfully applied to detect SCCA in human serum.
本研究旨在设计一种具有双重共反应加速剂的夹心型电化学发光（ECL）免疫传感器，以灵敏地检测鳞状细胞癌抗原（SCCA）。首先，将磷酸银（AgPO）纳米粒子修饰在 EuPO 纳米线上，以提高其较差的分散性/溶解性。同时，EuPO 被用作共反应加速剂来催化 SO 产生更多的中间产物（SO），显著增强了 AgPO 的 ECL 信号。AgPO@EuPO 复合纳米材料上修饰的 Ag 纳米粒子（AgNP）不仅可用作发光基团和生物标志物的连接体，还可用作共反应加速剂，有效地增强 ECL 信号。所设计的 ECL 免疫传感器具有稳定性和重现性好等优点。在最佳条件下，检测 SCCA 的线性范围为 0.0001-50ng·mL，检测限为 25fg·mL（S/N=3），回收率为 96.6-100.4%，相对标准偏差小于 4.8%。它成功地应用于检测人血清中的 SCCA。
