Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.
School of Pharmacy, Changzhou University, Changzhou 213164, China.
Analyst. 2021 Jun 28;146(13):4254-4260. doi: 10.1039/d1an00599e.
In this work, a novel electrochemiluminescence (ECL) aptasensor based on the resonance energy transfer (RET) effect between Ag3PO4-Cu-MOF (ii) and silver nanoparticles (Ag NPs) is proposed. The ECL emission spectra of Ag3PO4-Cu-MOF and the ultraviolet absorption spectra of Ag NPs showed a good spectral overlap. Based on this, we designed an "on-off-on" ECL sensing strategy for the sensitive and specific detection of diethylstilbestrol (DES). Under the optimal conditions, the linear range of the sensor for DES detection was 1.0 × 10-12-1.0 × 10-4 M, with a detection limit of 7.2 × 10-13 M (S/N = 3). The method showed simple and fast operation, high sensitivity and selectivity, a strong anti-interference ability and good stability. More importantly, the developed aptasensor exhibited excellent recognition towards residual DES in actual water samples. The sensor has superior measurement capability and potential application value in the field of environment water quality monitoring.
在这项工作中，提出了一种基于 Ag3PO4-Cu-MOF（ii）和银纳米粒子（Ag NPs）之间共振能量转移（RET）效应的新型电化学发光（ECL）适体传感器。Ag3PO4-Cu-MOF 的 ECL 发射光谱和 Ag NPs 的紫外吸收光谱具有良好的光谱重叠。基于此，我们设计了一种“开-关-开”ECL 传感策略，用于灵敏和特异性检测己烯雌酚（DES）。在最佳条件下，该传感器对 DES 的检测线性范围为 1.0×10-12-1.0×10-4 M，检测限为 7.2×10-13 M（S/N=3）。该方法具有操作简单、快速、灵敏度高、选择性好、抗干扰能力强、稳定性好等优点。更重要的是，所开发的适体传感器对实际水样中的残留 DES 表现出优异的识别能力。该传感器在环境水质监测领域具有卓越的测量能力和潜在的应用价值。
