Kurokochi Hiroyuki, Tajima Naoyuki, Sato Mitsuhiko P, Yoshitake Kazutoshi, Asakawa Shuichi, Isobe Sachiko, Shirasawa Kenta
Department of Forest Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
Department of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
DNA Res. 2023 Apr 25;30(3). doi: 10.1093/dnares/dsad006.
Here, we report the first telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake), which consists of 13 sequences (spanning 161.0 Mb) and a 76 kb circular mitochondrial genome. All the 13 sequences were supported with telomeric repeats at the ends. GC-rich regions are located at the middle of the sequences and are enriched with long interspersed nuclear elements (LINEs). Repetitive sequences including long-terminal repeats (LTRs) and LINEs occupy 71.6% of the genome. A total of 21,887 potential protein-coding genes were predicted. The genomic data reported in this study served not only matsutake gene sequences but also genome structures and intergenic sequences. The information gained would be a great reference for exploring the genetics, genomics, and evolutionary study of matsutake in the future, and ultimately facilitate the conservation of this vulnerable genetic resource.
在此，我们报告了松茸（松口蘑）的首个端粒到端粒的基因组组装结果，该基因组由13条序列（跨度为161.0 Mb）和一个76 kb的环状线粒体基因组组成。所有13条序列的末端均有端粒重复序列支持。富含GC的区域位于序列中间，并且富含长散在核元件（LINEs）。包括长末端重复序列（LTRs）和LINEs在内的重复序列占基因组的71.6%。共预测出21,887个潜在的蛋白质编码基因。本研究报告的基因组数据不仅提供了松茸的基因序列，还包括基因组结构和基因间序列。所获得的信息将为未来探索松茸的遗传学、基因组学和进化研究提供重要参考，并最终促进对这种脆弱遗传资源的保护。
