Tang Kuo-Shu, Tsai Chih-Min, Cheng Ming-Chou, Huang Ying-Hsien, Chang Chih-Hao, Yu Hong-Ren
Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan.
Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Taoyuan City 33302, Taiwan.
Diagnostics (Basel). 2023 Apr 18;13(8):1468. doi: 10.3390/diagnostics13081468.
Community-acquired pneumonia (CAP) is common among children and can be fatal in certain conditions. In children, CAP can be caused by viral or bacterial infections. Identification of pathogens can help select appropriate therapeutic strategies. Salivary analysis may be a potential diagnostic tool because it is noninvasive, patient-friendly, and easy to perform in children. A prospective study was conducted in children with pneumonia admitted to a hospital. Salivary samples from patients with definite and influenza A strains were used for gel-free (isobaric tag for relative and absolute quantitation (iTRAQ)) proteomics. No statistically significant difference was detected in salivary CRP levels between and influenza A pneumonia in children. Several potential salivary biomarkers were identified using gel-free iTRAQ proteomics to differentiate pneumonia from or influenza A virus infections in pediatric patients. ELISA validated that group has a higher abundance of salivary alpha 1-antichymotrypsin than those in the influenza A group. Whether these salivary biomarkers can be used to distinguish other bacteria from viral pneumonia requires further verification.
社区获得性肺炎（CAP）在儿童中很常见，在某些情况下可能致命。在儿童中，CAP可由病毒或细菌感染引起。病原体的鉴定有助于选择合适的治疗策略。唾液分析可能是一种潜在的诊断工具，因为它是非侵入性的，对患者友好，并且在儿童中易于操作。对一家医院收治的肺炎儿童进行了一项前瞻性研究。将确诊为[具体疾病]和甲型流感菌株患者的唾液样本用于无凝胶（相对和绝对定量等压标签（iTRAQ））蛋白质组学研究。儿童[具体疾病]肺炎和甲型流感肺炎患者的唾液CRP水平未检测到统计学上的显著差异。使用无凝胶iTRAQ蛋白质组学鉴定了几种潜在的唾液生物标志物，以区分儿科患者的[具体疾病]或甲型流感病毒感染与肺炎。酶联免疫吸附测定（ELISA）证实，[具体疾病]组唾液α1-抗糜蛋白酶的丰度高于甲型流感组。这些唾液生物标志物是否可用于区分其他细菌与病毒性肺炎尚需进一步验证。
