PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD, Montpellier, France.
PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD, Montpellier, France.
Curr Opin Plant Biol. 2023 Aug;74:102381. doi: 10.1016/j.pbi.2023.102381. Epub 2023 May 14.
The specific recognition of pathogen effectors by intracellular nucleotide-binding and leucine-rich repeat domain receptors (NLRs) is an important component of plant immunity. Creating NLRs with new bespoke recognition specificities is a major goal in molecular plant pathology as it promises to provide unlimited resources for the resistance of crops against diseases. Recent breakthrough discoveries on the structure and molecular activity of NLRs begin to enable their knowledge-guided molecular engineering. First, studies succeeded to extend or change effector recognition specificities by modifying, in a structure-guided manner, the NLR domains that directly bind effectors. By modifying the LRR domain of the singleton NLR Sr35 or the unconventional decoy domains of the helper NLRs RGA5 or Pik-1, receptors that detected other or additional effectors were created.
细胞内核苷酸结合和富含亮氨酸重复结构域受体（NLRs）对病原体效应物的特异性识别是植物免疫的一个重要组成部分。创造具有新的定制识别特异性的 NLRs 是分子植物病理学的主要目标，因为它有望为作物抵抗疾病提供无限的资源。最近关于 NLR 结构和分子活性的突破性发现开始使它们能够进行基于知识的分子工程。首先，通过以结构为导向的方式修饰直接结合效应物的 NLR 结构域，研究成功地扩展或改变了效应物识别特异性。通过修饰单体 NLR Sr35 的 LRR 结构域或辅助 NLRs RGA5 或 Pik-1 的非传统诱饵结构域，创建了可以检测其他或额外效应物的受体。
