Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, 1030, Austria.
Curr Opin Plant Biol. 2023 Aug;74:102380. doi: 10.1016/j.pbi.2023.102380. Epub 2023 May 13.
Factors including climate change and increased global exchange are set to escalate the prevalence of plant diseases, posing an unprecedented threat to global food security and making it more challenging to meet the demands of an ever-growing population. As such, new methods of pathogen control are essential to help with the growing danger of crop losses to plant diseases. The intracellular immune system of plants utilizes nucleotide-binding leucine-rich repeat (NLR) receptors to recognize and activate defense responses to pathogen virulence proteins (effectors) delivered to the host. Engineering the recognition properties of plant NLRs toward pathogen effectors is a genetic solution to plant diseases with high specificity, and it is more sustainable than several current methods for pathogen control that frequently rely on agrochemicals. Here, we highlight the pioneering approaches toward enhancing effector recognition in plant NLRs and discuss the barriers and solutions in engineering the plant intracellular immune system.
包括气候变化和全球交流增加在内的多种因素，预计将使植物疾病的流行率上升，这对全球粮食安全构成了前所未有的威胁，也使得满足不断增长的人口需求变得更加具有挑战性。因此，需要采用新的病原体控制方法来帮助应对因植物疾病而导致的作物损失日益增加的危险。植物的细胞内免疫系统利用核苷酸结合富含亮氨酸重复（NLR）受体来识别和激活对病原体毒力蛋白（效应子）的防御反应，这些效应子被递送到宿主中。针对病原体效应子对植物 NLR 的识别特性进行工程改造是一种具有高度特异性的植物疾病的遗传解决方案，而且比目前几种经常依赖农药的病原体控制方法更具可持续性。在这里，我们重点介绍了提高植物 NLR 中效应子识别的开创性方法，并讨论了在工程改造植物细胞内免疫系统方面的障碍和解决方案。
