Yang Juan, Qin Dongmiao, Wang Na, Wu Yusheng, Fang Kanjun, Deng Biyang
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Anal Chem. 2023 May 2;95(17):7045-7052. doi: 10.1021/acs.analchem.3c00729. Epub 2023 Apr 20.
A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid β42 (Aβ42). The synthesized silver nanoparticle-functionalized zinc metal-organic framework (Ag@ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH) were used as the ECL donor and acceptor, respectively. AgNPs were generated on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1). Under the optimized experimental conditions, the linear detection range of Aβ42 concentration was 10 fg/mL to 100 ng/mL, and the detection limit was 2.4 fg/mL (S/N = 3). The recoveries of Aβ42 were 99.5-104%. The method has good stability, repeatability, and specificity. Ag@ZnPTC/Au@UiO-66-NH provides an assay for the sensitive detection of disease biomarkers.
设计了一种基于聚集诱导电化学发光共振能量转移（AIECL-RET）的新型夹心式电化学发光（ECL）生物传感器，用于灵敏检测β淀粉样蛋白42（Aβ42）。合成的银纳米颗粒功能化锌金属有机框架（Ag@ZnPTC）和金纳米颗粒功能化锆有机框架（Au@UiO-66-NH）分别用作ECL供体和受体。在ZnPTC表面生成了AgNPs，这进一步提高了ECL强度和抗体1（Ab1）的负载量。在优化的实验条件下，Aβ42浓度的线性检测范围为10 fg/mL至100 ng/mL，检测限为2.4 fg/mL（S/N = 3）。Aβ42的回收率为99.5-104%。该方法具有良好的稳定性、重复性和特异性。Ag@ZnPTC/Au@UiO-66-NH为疾病生物标志物的灵敏检测提供了一种检测方法。
