miR-30a-5p 通过调控 CBX2 抑制食管癌细胞行为。
MiR-30a-5p inhibits cell behaviors in esophageal cancer via modulating CBX2.
机构信息
The Department of Radiotherapy the Center of Oncology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
The Department of Radiotherapy the Center of Oncology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
出版信息
Mutat Res. 2023 Jan-Jun;826:111818. doi: 10.1016/j.mrfmmm.2023.111818. Epub 2023 Apr 28.
BACKGROUND
This investigation studied the impacts of the miR-30a-5p/CBX2 axis on esophageal cancer (EC).
METHODS
Research objects were ascertained using The Cancer Genome Atlas database. Followed by qRT-PCR, western blot, dual-luciferase reporter, MTT, Transwell, and wound healing approaches, we tested gene expression and varying cell behaviors RESULTS: Conspicuously miR-30 family members (miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p) downregulation and CBX2 upregulation were discovered in EC cells. miR-30 family members target CBX2 and inhibited CBX2 expression. EC cell behaviors were inhibited by miR-30a-5p/CBX2 axis.
CONCLUSION
MiR-30a-5p draws a new inspiration for EC treatment.
背景
本研究探讨了 miR-30a-5p/CBX2 轴对食管癌（EC）的影响。
方法
利用癌症基因组图谱数据库确定研究对象。随后通过 qRT-PCR、western blot、双荧光素酶报告、MTT、Transwell 和划痕愈合实验，检测基因表达和各种细胞行为。
结果
在 EC 细胞中发现 miR-30 家族成员（miR-30a-5p、miR-30b-5p、miR-30c-5p、miR-30d-5p、miR-30e-5p）下调和 CBX2 上调。miR-30 家族成员靶向 CBX2 并抑制 CBX2 表达。miR-30a-5p/CBX2 轴抑制 EC 细胞行为。
结论
miR-30a-5p 为 EC 治疗提供了新的思路。
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