下调 miR-30a-3p/5p 通过激活 Wnt 信号通路促进食管鳞癌细胞增殖。

Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway.

机构信息

Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.

Periodicals Publishing House, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.

出版信息

World J Gastroenterol. 2017 Dec 7;23(45):7965-7977. doi: 10.3748/wjg.v23.i45.7965.

AIM

To investigate the potential role of microRNA-30a (miR-30a) in esophageal squamous cell carcinoma (ESCC).

METHODS

Expression of miR-30a-3p/5p was analyzed using microarray data and fresh ESCC tissue samples. Both and assays were used to investigate the effects of miR-30a-3p/5p on ESCC cell proliferation. Furthermore, Kyoto Encyclopedia of Genes and Genomes analysis was performed to explore underlying mechanisms involved in ESCC, and then, assays were carried out to verify the potential molecular mechanism of miR-30a in ESCC.

RESULTS

Low expression of miR-30a-3p/5p was closely associated with advanced ESCC progression and poor prognosis of patients with ESCC. Knock-down of miR-30a-3p/5p promoted ESCC cell proliferation. Increased miR-30a-3p/5p expression inhibited the Wnt signaling pathway by targeting Wnt2 and Fzd2.

CONCLUSION

Down-regulation of miR-30a-3p/5p promotes ESCC cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2.

目的

探讨微小 RNA-30a（miR-30a）在食管鳞状细胞癌（ESCC）中的潜在作用。

方法

使用微阵列数据和新鲜的 ESCC 组织样本分析 miR-30a-3p/5p 的表达。使用和测定法来研究 miR-30a-3p/5p 对 ESCC 细胞增殖的影响。此外，进行京都基因与基因组百科全书分析以探讨 ESCC 涉及的潜在机制，然后进行测定法以验证 miR-30a 在 ESCC 中的潜在分子机制。