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通过筛选建立具有网膜嗜性的高转移性卵巢癌模型。

Establishment of highly metastatic ovarian cancer model with omental tropism via selection.

作者信息

Ying Feiquan, Guo Jing, Gao Xuejiao, Huang Lin, Gao Lingling, Cai Jing, Wang Zehua

机构信息

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

出版信息

iScience. 2023 Apr 23;26(5):106719. doi: 10.1016/j.isci.2023.106719. eCollection 2023 May 19.

DOI:10.1016/j.isci.2023.106719
PMID:37197325
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10183668/
Abstract

Epithelial ovarian cancer (OC) is often diagnosed at an advanced stage with peritoneal metastasis, and preclinical models mimicking the natural course of OC peritoneal metastasis are essential to improve treatment. We implanted ES2 and ID8 cells in the ovaries of mice and obtained highly metastatic (HM) sublines from their omental metastases after three cycles selection. Orthotopic xenografts derived from the HM sublines showed enhanced omental tropism and more extensive metastasis with earlier onset. The HM cells exhibited increased migration and invasion properties, and RNA sequencing revealed that the genes related to epithelial-mesenchymal transition and extracellular matrix regulation were significantly altered in the HM cells. Among them, the upregulated genes were significantly associated with poorer survival in OC patients. In conclusion, these HM sublines can be leveraged to establish spontaneous metastatic OC mouse models, which may serve as ideal preclinical models for anti-metastasis therapy for OC patients.

摘要

上皮性卵巢癌（OC）通常在出现腹膜转移时才被诊断为晚期，而模拟OC腹膜转移自然病程的临床前模型对于改善治疗至关重要。我们将ES2和ID8细胞植入小鼠卵巢，并经过三个周期的筛选后从其网膜转移灶中获得了高转移性（HM）亚系。源自HM亚系的原位异种移植显示出更强的网膜嗜性，转移更广泛且发病更早。HM细胞表现出增强的迁移和侵袭特性，RNA测序显示，与上皮-间质转化和细胞外基质调节相关的基因在HM细胞中发生了显著改变。其中，上调的基因与OC患者较差的生存率显著相关。总之，这些HM亚系可用于建立自发性转移性OC小鼠模型，这可能成为OC患者抗转移治疗的理想临床前模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/fd6dec751c25/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/3b22a8f8ba86/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/de638288add2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/69c7222ae669/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/2c8c42b17e60/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/e9f6f9b10021/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/fd6dec751c25/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/3b22a8f8ba86/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/de638288add2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/69c7222ae669/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/2c8c42b17e60/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/e9f6f9b10021/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a10a/10183668/fd6dec751c25/gr5.jpg

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本文引用的文献

1
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CA Cancer J Clin. 2023 Jan;73(1):17-48. doi: 10.3322/caac.21763.
2
Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2022.2022 年中国国家生物信息中心国家基因组学数据中心数据库资源。
Nucleic Acids Res. 2022 Jan 7;50(D1):D27-D38. doi: 10.1093/nar/gkab951.
3
Assessment of the immune landscapes of advanced ovarian cancer in an optimized in vivo model.优化体内模型评估晚期卵巢癌的免疫图谱。
异质性核糖核蛋白A2B1通过将货物分选到驱动肌成纤维细胞激活的小细胞外囊泡中促进卵巢癌转移。
J Nanobiotechnology. 2025 Apr 4;23(1):273. doi: 10.1186/s12951-025-03342-w.
4
Exosomal ANXA2 facilitates ovarian cancer peritoneal metastasis by activating peritoneal mesothelial cells through binding with TLR2.外泌体膜联蛋白A2通过与Toll样受体2（TLR2）结合激活腹膜间皮细胞，促进卵巢癌腹膜转移。
Cell Commun Signal. 2024 Dec 21;22(1):616. doi: 10.1186/s12964-024-01987-y.
5
Tumor-derived small extracellular vesicles facilitate omental metastasis of ovarian cancer by triggering activation of mesenchymal stem cells.肿瘤来源的小细胞外囊泡通过触发间充质干细胞的激活促进卵巢癌的大网膜转移。
Cell Commun Signal. 2024 Jan 17;22(1):47. doi: 10.1186/s12964-023-01413-9.
Clin Transl Med. 2021 Oct;11(10):e551. doi: 10.1002/ctm2.551.
4
The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types.基因组序列档案家族：走向爆炸式的数据增长和多样化的数据类型。
Genomics Proteomics Bioinformatics. 2021 Aug;19(4):578-583. doi: 10.1016/j.gpb.2021.08.001. Epub 2021 Aug 13.
5
Ovarian Cancer Immunotherapy and Personalized Medicine.卵巢癌免疫治疗与个性化医学。
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J Clin Oncol. 2021 Jun 10;39(17):1842-1855. doi: 10.1200/JCO.21.00306. Epub 2021 Apr 23.
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Clin Cancer Res. 2021 Sep 15;27(18):4953-4973. doi: 10.1158/1078-0432.CCR-20-2833. Epub 2021 Apr 22.
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The In Vivo Selection Method in Breast Cancer Metastasis.体内乳腺癌转移的选择方法。
Int J Mol Sci. 2021 Feb 14;22(4):1886. doi: 10.3390/ijms22041886.