Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
Analytical & Testing Center, Sichuan University, Chengdu 610064, China.
Anal Chem. 2023 May 30;95(21):8310-8317. doi: 10.1021/acs.analchem.3c00738. Epub 2023 May 18.
Chemiluminescence (CL) probes that possess near-infrared (NIR) emission are highly desirable for in vivo imaging due to their deeper tissue penetration ability and intrinsically high sensitivity. Herein, a novel iridium-based CL probe () with direct NIR emission was reported as the result of hypochlorous acid (HClO)-initiated oxidative deoximation. To improve its biocompatibility and extend the CL time for in vivo imaging applications, this was prepared as a CL nanoparticle probe (s) through encapsulation by an amphiphilic polymer Pluronic F127 (F127). All results demonstrate that the have good selectivity and sensitivity for visualization of HClO even at the depth of 1.2 cm. Owing to these advantages, the CL imaging of exogenous and endogenous HClO in mice was achieved. This study could provide new insights into the construction of new NIR emission CL probes and expand their applications in biomedical imaging.
化学发光（CL）探针具有近红外（NIR）发射，由于其更深的组织穿透能力和固有的高灵敏度，非常适用于体内成像。在此，报道了一种新型基于铱的 CL 探针（），它具有直接的近红外发射，这是次氯酸（HClO）引发的氧化脱氨作用的结果。为了提高其生物相容性并延长体内成像应用的 CL 时间，通过两亲性聚合物 Pluronic F127（F127）包封，将该探针制备成 CL 纳米颗粒探针（s）。所有结果均表明，即使在 1.2 cm 的深度，探针也对 HClO 的可视化具有良好的选择性和灵敏度。由于这些优势，成功实现了小鼠中外源和内源性 HClO 的 CL 成像。本研究可为构建新型近红外发射 CL 探针提供新的见解，并扩展其在生物医学成像中的应用。
