State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.
Co-Innovation Center for Sustainable Forestry in Southern China/Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China.
Plant Sci. 2021 Oct;311:111021. doi: 10.1016/j.plantsci.2021.111021. Epub 2021 Aug 14.
HD-Zip is a plant-specific HB transcription factor, which participates in plant development and stress response. In this study, we identified 63 poplar HD-Zip transcription factors, which were randomly distributed on 19 chromosomes of poplar. Based on the gene structure and phylogenetic relationship, these members are divided into four groups, which have a variety of collinear evolutionary relationships. They also have rich segmental replication events and experienced strong purification selection. Based on RNA-seq analysis, we profiled the expression pattern of the 63 HD-Zip members under salt stress. Subsequently, we carried out in-depth study on the significantly up-regulated PsnHDZ63 in the stems and leaves. The transgenic Populus simonii × P.nigra plants over-expressing PsnHDZ63 displayed better morphological and physiological indexes than WT under salt stress. In addition, PsnHDZ63 enhanced salt stress tolerance of transgenic lines by combining effective stress-resistant elements to improve reactive oxygen species scavenging ability. These studies laid a foundation for a comprehensive understanding of poplar HD-Zip family members, and revealed the important role of PsnHDZ63 in plant salt tolerance.
HD-Zip 是一种植物特异性的 HB 转录因子，参与植物的发育和应激反应。本研究鉴定了 63 个杨树 HD-Zip 转录因子，它们随机分布在杨树的 19 条染色体上。基于基因结构和系统发育关系，这些成员被分为四个组，它们具有多种共线性进化关系。它们还具有丰富的片段复制事件，并经历了强烈的纯化选择。基于 RNA-seq 分析，我们对 63 个 HD-Zip 成员在盐胁迫下的表达模式进行了分析。随后，我们对茎和叶中显著上调的 PsnHDZ63 进行了深入研究。过表达 PsnHDZ63 的转基因 Populus simonii × P.nigra 植物在盐胁迫下表现出比 WT 更好的形态和生理指标。此外，PsnHDZ63 通过结合有效的应激抗性元件来提高活性氧清除能力，增强了转基因株系的耐盐性。这些研究为全面了解杨树 HD-Zip 家族成员奠定了基础，并揭示了 PsnHDZ63 在植物耐盐性中的重要作用。
