Sanya Nanfan Research Institute, College of Food Science and Engineering, College of Plant Protection, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, PR China; Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, PR China.
Sanya Nanfan Research Institute, College of Food Science and Engineering, College of Plant Protection, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, PR China.
Environ Pollut. 2023 Aug 15;331(Pt 1):121839. doi: 10.1016/j.envpol.2023.121839. Epub 2023 May 17.
Acetamiprid (ACE) and cyromazine (CYR) are the two pesticides that are used relatively frequently and in large quantities in cowpea growing areas in Hainan. The uptake, translocation and metabolic patterns and subcellular distribution of these two pesticides in cowpea are important factors affecting pesticide residues in cowpea and assessing the dietary safety of cowpea. In this study, we investigated the uptake, translocation, subcellular distribution, and metabolic pathway of ACE and CYR in cowpea under laboratory hydroponic conditions. The distribution trends of both ACE and CYR in cowpea plants were leaves > stems > roots. The distribution of both pesticides in subcellular tissues of cowpea was cell soluble fraction > cell wall > cell organelle, and both transport modes were passive. A multiplicity of metabolic reactions of both pesticides occurred in cowpea, including dealkylation, hydroxylation and methylation. The results of the dietary risk assessment indicate that ACE is safe for use in cowpeas, but CYR poses an acute dietary risk to infants and young children. This study provided a basis for insights into the transport and distribution of ACE and CYR in vegetables and contributes to the assessment of whether pesticide residues in vegetables could pose a potential threat to human health at high concentrations of pesticides in the environment.
乙酰甲胺磷（ACE）和环丙氨嗪（CYR）是在海南豇豆种植区使用相对频繁和大量的两种农药。这两种农药在豇豆中的吸收、转运和代谢模式以及亚细胞分布是影响豇豆中农药残留和评估豇豆膳食安全性的重要因素。本研究采用室内水培条件，研究了 ACE 和 CYR 在豇豆中的吸收、转运、亚细胞分布和代谢途径。ACE 和 CYR 在豇豆植株中的分布趋势为叶片＞茎＞根。两种农药在豇豆亚细胞组织中的分布为胞质可溶性部分＞细胞壁＞细胞器官，两种运输方式均为被动运输。两种农药在豇豆中发生了多种代谢反应，包括脱烷基化、羟化和甲基化。膳食风险评估结果表明，ACE 可安全用于豇豆，但 CYR 对婴幼儿构成急性膳食风险。本研究为了解 ACE 和 CYR 在蔬菜中的运输和分布提供了依据，有助于评估在环境中高浓度农药的情况下，蔬菜中的农药残留是否会对人类健康构成潜在威胁。
