Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs; Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables; Key Laboratory of Nutritional Quality and Health Benefits of Tropical Agricultural Products of Haikou City, Haikou 571101, Hainan, China; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China.
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs; Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables; Key Laboratory of Nutritional Quality and Health Benefits of Tropical Agricultural Products of Haikou City, Haikou 571101, Hainan, China.
J Hazard Mater. 2024 Jul 5;472:134534. doi: 10.1016/j.jhazmat.2024.134534. Epub 2024 May 7.
Cowpea plants, renowned for their high edibility, pose a significant risk of pesticide residue contamination. Elucidating the behavior of pesticide residues and their key metabolic pathways is critical for ensuring cowpea safety and human health. This study investigated the migration of pesticide residues and their key metabolic pathways in pods throughout the growth process of cowpea plants via in situ mass spectrometry. To this end, four pesticides--including systemic (thiram), and nonsystemic (fluopyram, pyriproxyfen, and cyromazine) pesticides--were selected. The results indicate the direct upward and downward transmission of pesticides in cowpea stems and pods. Systemic pesticides gradually migrate to the core of cowpea plants, whereas nonsystemic pesticides remain on the surface of cowpea peels. The migration rate is influenced by the cowpea maturity, logarithmic octanol-water partition coefficient (log Kow) value, and molecular weight of the pesticide. Further, 20 types of key metabolites related to glycolysis, tricarboxylic acid cycle, and flavonoid synthesis were found in cowpea pods after pesticide treatment. These findings afford insights into improving cowpea quality and ensuring the safe use of pesticides.
菜豆植株以其高食用性而闻名，但存在农药残留污染的重大风险。阐明农药残留的行为及其关键代谢途径对于确保菜豆安全和人类健康至关重要。本研究通过原位质谱法研究了菜豆植株生长过程中豆荚中农药残留及其关键代谢途径的迁移。为此，选择了四种农药 - 包括内吸性（福美双）和非内吸性（氟吡菌胺、吡虫啉和环丙氨嗪）农药。结果表明，农药在菜豆茎和豆荚中直接向上和向下传输。内吸性农药逐渐迁移到菜豆植物的核心，而非内吸性农药则停留在菜豆皮的表面。迁移率受菜豆成熟度、辛醇-水分配系数（log Kow）值和农药分子量的影响。此外，在菜豆荚经过农药处理后，发现了 20 种与糖酵解、三羧酸循环和类黄酮合成相关的关键代谢物。这些发现为提高菜豆质量和确保农药安全使用提供了依据。
