Department of Gastroenterology, Jinhua People's Hospital, Jinhua 321000, Zhejiang Province, China.
Department of Hepatobiliary and Pancreatic Gastroenterology, Jinhua People's Hospital, Jinhua 321000, Zhejiang Province, China.
Genomics. 2023 Jul;115(4):110641. doi: 10.1016/j.ygeno.2023.110641. Epub 2023 May 16.
Gastric cancer (GC) is a common cancer with a high incidence and mortality rate. Herein, the role of hsa_circ_0002019 (circ_0002019) in GC was investigated.
The molecular structure and stability of circ_0002019 were identified by RNase R, and Actinomycin D treatment. Molecular associations were verified by RIP. Proliferation, migration, and invasion were detected by CCK-8, EdU, and Transwell, respectively. The effect of circ_0002019 on tumor growth was analyzed in vivo.
Circ_0002019 was elevated in GC tissues and cells. Circ_0002019 knockdown inhibited the proliferation, migration, and invasion. Mechanically, circ_0002019 activated NF-κB signaling by increasing TNFAIP6 mRNA stability by PTBP1. Activation of NF-κB signaling limited the antitumor effect of circ_0002019 silencing in GC. Circ_0002019 knockdown inhibited tumor growth in vivo by reducing TNFAIP6 expression.
Circ_0002019 accelerated the proliferation, migration, and invasion by regulating TNFAIP6/NF-κB pathway, suggesting circ_0002019 could be a key regulatory factor in GC progression.
胃癌（GC）是一种常见的癌症，发病率和死亡率都很高。在此，研究了 hsa_circ_0002019（circ_0002019）在 GC 中的作用。
通过 RNase R 和 Actinomycin D 处理鉴定 circ_0002019 的分子结构和稳定性，并通过 RIP 验证分子关联。通过 CCK-8、EdU 和 Transwell 分别检测增殖、迁移和侵袭。在体内分析 circ_0002019 对肿瘤生长的影响。
circ_0002019 在 GC 组织和细胞中升高。circ_0002019 敲低抑制增殖、迁移和侵袭。机制上，circ_0002019 通过增加 PTBP1 介导的 TNFAIP6 mRNA 稳定性来激活 NF-κB 信号。NF-κB 信号的激活限制了 circ_0002019 沉默在 GC 中的抗肿瘤作用。circ_0002019 敲低通过降低 TNFAIP6 表达抑制体内肿瘤生长。
circ_0002019 通过调节 TNFAIP6/NF-κB 通路加速增殖、迁移和侵袭，表明 circ_0002019 可能是 GC 进展的关键调节因子。
