Laboratory of Biological Characterization of Advanced Materials (LBCAM), Trinity Translational Medicine Institute, Trinity College, Dublin, Ireland.
Nanomedicine and Molecular Imaging Group, Trinity Translational Medicine Institute (TTMI), School of Medicine, Trinity College Dublin, Dublin, Ireland.
Methods Mol Biol. 2023;2645:3-40. doi: 10.1007/978-1-0716-3056-3_1.
Despite significant advances in investigative and therapeutic methodologies for cancer, 2D cell culture remains an essential and evolving competency in this fast-paced industry. From basic monolayer cultures and functional assays to more recent and ever-advancing cell-based cancer interventions, 2D cell culture plays a crucial role in cancer diagnosis, prognosis, and treatment. Research and development in this field call for a great deal of optimization, while the heterogenous nature of cancer itself demands personalized precision for its intervention. In this way, 2D cell culture is ideal, providing a highly adaptive and responsive platform, where skills can be honed and techniques modified. Furthermore, it is arguably the most efficient, economical, and sustainable methodology available to researchers and clinicians alike.In this chapter, we discuss the history of cell culture and the varying types of cell and cell lines used today, the techniques used to characterize and authenticate them, the applications of 2D cell culture in cancer diagnosis and prognosis, and more recent developments in the area of cell-based cancer interventions and vaccines.
尽管癌症的研究和治疗方法取得了重大进展，但二维细胞培养仍然是这个快速发展的行业中必不可少且不断发展的能力。从基本的单层培养和功能测定，到最近和不断发展的基于细胞的癌症干预，二维细胞培养在癌症诊断、预后和治疗中发挥着关键作用。该领域的研究和开发需要大量的优化，而癌症本身的异质性要求对其进行个性化的精准干预。在这方面，二维细胞培养是理想的，它提供了一个高度适应和响应的平台，可以磨练技能和修改技术。此外，可以说，对于研究人员和临床医生来说，这是最有效、最经济和最可持续的方法。在本章中，我们讨论了细胞培养的历史以及当今使用的各种类型的细胞和细胞系，用于对其进行表征和验证的技术，二维细胞培养在癌症诊断和预后中的应用，以及细胞基癌症干预和疫苗领域的最新进展。
