Houser Aubrey E, Kazmi Abiha, Nair Arjun K, Ji Andrew L
Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
JID Innov. 2023 Mar 22;3(4):100198. doi: 10.1016/j.xjidi.2023.100198. eCollection 2023 Jul.
The development of multiomic profiling tools has rapidly expanded in recent years, along with their use in profiling skin tissues in various contexts, including dermatologic diseases. Among these tools, single-cell RNA-sequencing (scRNA-seq) and spatial transcriptomics (ST) have emerged as widely adopted and powerful assays for elucidating key cellular components and their spatial arrangement within skin disease. In this paper, we review the recent biological insights gained from the use of scRNA-seq and ST and the advantages of combining both for profiling skin diseases, including aberrant wound healing, inflammatory skin diseases, and cancer. We discuss the role of scRNA-seq and ST in improving skin disease treatments and moving toward the goal of achieving precision medicine in dermatology, whereby patients can be optimally matched to treatments that maximize therapeutic response.
近年来，多组学分析工具发展迅速，其在包括皮肤病在内的各种情况下对皮肤组织进行分析的应用也不断增加。在这些工具中，单细胞RNA测序（scRNA-seq）和空间转录组学（ST）已成为广泛采用且强大的分析方法，用于阐明皮肤疾病中关键细胞成分及其空间排列。在本文中，我们回顾了使用scRNA-seq和ST所获得的最新生物学见解，以及将两者结合用于分析皮肤疾病（包括异常伤口愈合、炎症性皮肤病和癌症）的优势。我们讨论了scRNA-seq和ST在改善皮肤病治疗以及朝着实现皮肤病精准医学目标迈进方面的作用，通过精准医学，患者可以与能使治疗反应最大化的治疗方法实现最佳匹配。
