Kim Sung-Won, Im Gwang-Bum, Kim Yeong Hwan, Bhang Suk Ho
School of Chemical Engineering, Sungkyunkwan University Suwon South Korea.
Present address: Department of Cardiac Surgery Boston Children's Hospital and Harvard Medical School Boston Massachusetts USA.
Bioeng Transl Med. 2022 Dec 8;8(3):e10462. doi: 10.1002/btm2.10462. eCollection 2023 May.
We used a blue organic light-emitting diode (bOLED) to increase the paracrine factors secreted from human adipose-derived stem cells (hADSCs) for producing conditioned medium (CM). Our results showed that while the bOLED irradiation promotes a mild-dose reactive oxygen generation that enhances the angiogenic paracrine secretion of hADSCs, it does not induce phototoxicity. The bOLED enhances paracrine factors via a cell-signaling mechanism involving hypoxia-inducible factor 1 alpha. This study demonstrated that the CM resulting from bOLED treatment shows improved therapeutic effects on mouse wound-healing models. This method contributes to overcoming the barriers to stem-cell therapies, including the toxicity and low yields from other methods such as nanoparticles, synthetic polymers, and even cell-derived vesicles.
我们使用蓝色有机发光二极管（bOLED）来增加人脂肪来源干细胞（hADSCs）分泌的旁分泌因子，以制备条件培养基（CM）。我们的结果表明，虽然bOLED照射会促进适度剂量的活性氧生成，从而增强hADSCs的血管生成旁分泌分泌，但它不会诱导光毒性。bOLED通过涉及缺氧诱导因子1α的细胞信号传导机制增强旁分泌因子。这项研究表明，bOLED处理产生的CM对小鼠伤口愈合模型具有更好的治疗效果。这种方法有助于克服干细胞疗法的障碍，包括纳米颗粒、合成聚合物甚至细胞衍生囊泡等其他方法的毒性和低产量问题。
