School of Chemical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon, 14662, Gyeonggi, Republic of Korea.
Commun Biol. 2022 Sep 13;5(1):957. doi: 10.1038/s42003-022-03838-3.
Conditioned medium (CM) contains various therapeutic molecules produced by cells. However, the low concentration of therapeutic molecules in CM is a major challenge for successful tissue regeneration. Here, we aim to develop a CM enriched in angiogenic paracrine factors for the treatment of ischemic diseases. Combining spheroidal culture and light irradiation significantly upregulates the angiogenic factor expression in human adipose-derived stem cells (hADSCs). Spheroids of light-irradiated hADSCs (SR group) show significantly enhanced expression of angiogenic paracrine factors compared with spheroids without light stimulation. Enhanced viability, migration, and angiogenesis are observed in cells treated with CM derived from the SR group. Furthermore, we performed in vivo experiments using a mouse hindlimb ischemia model; the results demonstrate that CM derived from densely cultured spheroids of light-irradiated hADSCs induced increased angiogenesis in vivo. In conclusion, our proposed approach of using light to stimulate stem cells may overcome the major drawbacks of CM-based therapies.
条件培养基（CM）中含有各种由细胞产生的治疗分子。然而，CM 中治疗分子的浓度低是成功进行组织再生的主要挑战。在这里，我们旨在开发富含血管生成旁分泌因子的 CM，用于治疗缺血性疾病。球形培养和光照射的结合显著上调了人脂肪来源干细胞（hADSCs）中血管生成因子的表达。与未经光刺激的球体相比，光照射的 hADSCs 球体（SR 组）显示出明显增强的血管生成旁分泌因子表达。来自 SR 组的 CM 处理的细胞表现出增强的活力、迁移和血管生成。此外，我们使用小鼠后肢缺血模型进行了体内实验；结果表明，来自光照射的 hADSCs 密集培养的球体衍生的 CM 诱导了体内血管生成的增加。总之，我们提出的使用光刺激干细胞的方法可能克服 CM 为基础的治疗方法的主要缺点。
