Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove, Brisbane, QLD, 4059, Australia; UQ Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, QLD, 4102, Australia; Herston Biofabrication Institute, Metro North Hospital and Health Service, Brisbane, QLD, 4029, Australia.
Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove, Brisbane, QLD, 4059, Australia.
Biomaterials. 2021 Jan;268:120558. doi: 10.1016/j.biomaterials.2020.120558. Epub 2020 Nov 27.
Biomimetically designed medical-grade polycaprolactone (mPCL) dressings are 3D-printed with pore architecture and anisotropic mechanical characteristics that favor skin wound healing with reduced scarring. Melt electrowritten mPCL dressings are seeded with human gingival tissue multipotent mesenchymal stem/stromal cells and cryopreserved using a clinically approved method. The regenerative potential of fresh or frozen cell-seeded mPCL dressing is compared in a splinted full-thickness excisional wound in a rat model over six weeks. The application of 3D-printed mPCL dressings decreased wound contracture and significantly improved skin regeneration through granulation and re-epithelialization compared to control groups. Combining 3D-printed biomimetic wound dressings and precursor cell delivery enhances physiological wound closure with reduced scar tissue formation.
仿生设计的医疗级聚己内酯（mPCL）敷料采用 3D 打印技术制成，具有孔隙结构和各向异性机械特性，有利于皮肤伤口愈合，减少瘢痕形成。熔融电写入的 mPCL 敷料接种了人牙龈组织多能间充质干细胞，并采用临床认可的方法进行冷冻保存。在六周的时间里，在大鼠模型的带夹板全层切除伤口中比较了新鲜或冷冻细胞接种的 mPCL 敷料的再生潜力。与对照组相比，3D 打印 mPCL 敷料的应用减少了伤口收缩，并通过肉芽形成和再上皮化显著改善了皮肤再生。结合 3D 打印仿生伤口敷料和前体细胞输送可增强生理性伤口闭合，减少瘢痕组织形成。
