Trulson M E, Arasteh K
Eur J Pharmacol. 1986 May 13;124(1-2):189-92. doi: 10.1016/0014-2999(86)90143-3.
Morphine produced a dose-dependent increase in the activity of norepinephrine-containing locus coeruleus neurons recorded from mouse brain slices in vitro. The response was not changed in a low calcium-high magnesium incubation medium, indicating that the observed effects are the result of a direct action of morphine on locus coeruleus neurons. When the mice were anesthetized with chloral hydrate prior to preparation of tissue slices, morphine produced a dose-dependent suppression of noradrenergic neuronal activity. These studies suggest that previous data showing a decrease in activity of locus coeruleus neurons following incubation with morphine in vitro are due to the use of anesthesia.
吗啡使体外记录的来自小鼠脑片的含去甲肾上腺素的蓝斑神经元活性呈剂量依赖性增加。在低钙 - 高镁孵育培养基中该反应未改变，表明观察到的效应是吗啡对蓝斑神经元直接作用的结果。当在制备组织切片之前用氯水合醛麻醉小鼠时，吗啡产生剂量依赖性的去甲肾上腺素能神经元活性抑制。这些研究表明，先前显示在体外与吗啡孵育后蓝斑神经元活性降低的数据是由于使用了麻醉。
