Luo Yinyue, Xiao Menglin, Almaqrami Bushra Sufyan, Kang Hong, Shao Zhengzhong, Chen Xin, Zhang Ying
Department of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai, 200001, China.
Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, 200002, China.
Biomater Res. 2023 May 19;27(1):50. doi: 10.1186/s40824-023-00370-1.
Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair's success.
Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with n-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments.
A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are developed and confirmed to promote chondrocyte migration and differentiation. Thus, osteochondral repair and superior horizontal integration are achieved in vivo with this composite.
Overall, the new approach of marginal sealing around the RSF scaffolds exhibits preeminent repair results, confirming the ability of this novel graft to facilitate simultaneous regeneration of cartilage-subchondral bone.
骨软骨缺损带来了巨大挑战，迄今为止尚无令人满意的修复策略。特别是，新生软骨与周围天然软骨的侧向整合是一个困难且未得到充分解决的问题，它决定了组织修复的成功与否。
创新性地用正丁醇制备了基于小孔径支架的再生丝素蛋白（RSF）。然后，将兔膝关节软骨细胞和骨髓间充质干细胞（BMSCs）接种于RSF支架上，诱导软骨分化后，制备用14 wt% RSF溶液强化的细胞支架复合物用于体内实验。
开发出一种具有生物相容性和优异粘附性能的多孔支架及RSF密封剂，并证实其可促进软骨细胞迁移和分化。因此，利用这种复合材料在体内实现了骨软骨修复及良好的水平整合。
总体而言，围绕RSF支架进行边缘密封的新方法显示出卓越的修复效果，证实了这种新型移植物促进软骨-软骨下骨同时再生的能力。
