通过低温3D打印制造的包含生长分化因子5（GDF-5）的分层多孔细胞外基质支架，以促进关节软骨再生。

Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration.

作者信息

Wu Jiang, Fu Liwei, Yan Zineng, Yang Yu, Yin Han, Li Pinxue, Yuan Xun, Ding Zhengang, Kang Teng, Tian Zhuang, Liao Zhiyao, Tian Guangzhao, Ning Chao, Li Yuguo, Sui Xiang, Chen Mingxue, Liu Shuyun, Guo Quanyi

机构信息

Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic of China.

Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, Institute of Orthopedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.

出版信息

Biomater Res. 2023 Feb 5;27(1):7. doi: 10.1186/s40824-023-00349-y.

BACKGROUND

In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more endogenous stem cells from the joint cavity to the defect area to promote cartilage regeneration.

METHOD

In this study, we used ECM alone as a bioink in low-temperature deposition manufacturing (LDM) 3D printing and then successfully fabricated a hierarchical porous ECM scaffold incorporating GDF-5.

RESULTS

Comparative in vitro experiments showed that the 7% ECM scaffolds had the best biocompatibility. After the addition of GDF-5 protein, the ECM scaffolds significantly improved bone marrow mesenchymal stem cell (BMSC) migration and chondrogenic differentiation. Most importantly, the in vivo results showed that the ECM/GDF-5 scaffold significantly enhanced in situ cartilage repair.

CONCLUSION

In conclusion, this study reports the construction of a new scaffold based on the concept of in situ regeneration, and we believe that our findings will provide a new treatment strategy for AC defect repair.