Chatterji D
FEBS Lett. 1986 Jul 14;203(1):54-8. doi: 10.1016/0014-5793(86)81435-1.
Poly(dG-dC) in 60% aqueous alcohol exhibits the characteristic inversion of the circular dichroism spectrum associated with the formation of left-handed helix. Upon complexation with Tb3+, poly(dG-dC) in this medium induces marked enhancement of the Tb(III) fluorescence emission at 488 and 545 nm, when excited at 290 nm. The degree of fluorescence enhancement is dependent on the concentration of Tb(III) at a fixed poly(dG-dC) concentration. Neither poly(dG-dC) in water nor poly(dA-dT) in water or 60% alcohol, causes any significant fluorescence spectral changes of Tb3+. Tb(III)-poly(dG-dC) in 60% alcohol shows circular dichroic spectra associated with a broad positive molar ellipticity ranging from 6000 to 10 000 degree X cm2 X dmol-1 between 270 and 280 nm, and a small negative band around 240 nm.
聚（dG-dC）在60%的乙醇水溶液中呈现出与左手螺旋形成相关的圆二色光谱特征性反转。在与Tb3+络合时，该介质中的聚（dG-dC）在290nm激发时，会使Tb(III)在488和545nm处的荧光发射显著增强。在固定的聚（dG-dC）浓度下，荧光增强程度取决于Tb(III)的浓度。水中的聚（dG-dC）以及水中或60%乙醇中的聚（dA-dT）均不会引起Tb3+的任何显著荧光光谱变化。60%乙醇中的Tb(III)-聚（dG-dC）显示出圆二色光谱，在270至280nm之间有一个宽的正摩尔椭圆率，范围为6000至10000度×厘米2×dmol-1，在240nm左右有一个小的负带。
