六氨合钌（III）氯化物：聚（dG-m5dC）·聚（dG-m5dC）和聚（dG-dC）·聚（dG-dC）从B-DNA向Z-DNA转变的高效促进剂。
Hexammineruthenium (III) chloride: a highly efficient promoter of the B-DNA to Z-DNA transition of poly-(dG-m5dC).poly(dG-m5dC) and poly(dG-dC).poly(dG-dC).
作者信息
Thomas T J, Messner R P
机构信息
Department of Medicine, University of Minnesota, Minneapolis 55455.
出版信息
Biochimie. 1988 Feb;70(2):221-6. doi: 10.1016/0300-9084(88)90064-8.
The effects of Ru(NH3)(3+)6 on the conformation of poly(dG-m5dC).poly(dG-m5dC) and poly(dG-dC).poly(dG-dC) were studied by circular dichroism (CD) spectroscopy. Ru(NH3)(3+)6 at very low concentrations provokes the Z-DNA conformation in both polynucleotides. In the presence of 50 mM NaCl, the concentration of Ru(NH3)(3+)6 at the midpoint of B to Z transition of poly(dG-m5dC).poly(dG-m5dC) is 4 microM compared to 5 microM for Co(NH3)(3+)6. The half-lives of B to Z transition of poly(dG-m5dC).poly(dG-m5dC) in the presence of 10 microM Ru(NH3)(3+)6 and Co(NHG3)(3+)6 are at 23 and 30 min, respectively. The concentration of Ru(NH3)(3+)6 at the midpoint of B to Z transition of poly(dG-dC).poly(dG-dC) is 50 microM. These results demonstrate that Ru(NH3)(3+)6 is a highly efficient trivalent cation for the induction of B to Z transition in poly(dG-m5dC).poly(dG-m5dC) and poly(dG-dC).poly(dG-dC). In contrast, Ru(NH3)(3+)6 has no significant effect on the conformation of calf thymus DNA, poly(dA-dT).poly(dA-dT) and poly(dA-dC).poly(dG-dT).
通过圆二色光谱法研究了六氨合钌（III）（Ru(NH₃)₆³⁺）对聚（dG-m⁵dC）·聚（dG-m⁵dC）和聚（dG-dC）·聚（dG-dC）构象的影响。极低浓度的Ru(NH₃)₆³⁺能诱导这两种多核苷酸形成Z-DNA构象。在50 mM NaCl存在的情况下，聚（dG-m⁵dC）·聚（dG-m⁵dC）从B型向Z型转变中点时Ru(NH₃)₆³⁺的浓度为4 μM，而六氨合钴（III）（Co(NH₃)₆³⁺）为5 μM。在10 μM Ru(NH₃)₆³⁺和Co(NH₃)₆³⁺存在时，聚（dG-m⁵dC）·聚（dG-m⁵dC）从B型向Z型转变的半衰期分别为23分钟和30分钟。聚（dG-dC）·聚（dG-dC）从B型向Z型转变中点时Ru(NH₃)₆³⁺的浓度为50 μM。这些结果表明，Ru(NH₃)₆³⁺是诱导聚（dG-m⁵dC）·聚（dG-m⁵dC）和聚（dG-dC）·聚（dG-dC）从B型向Z型转变的高效三价阳离子。相比之下，Ru(NH₃)₆³⁺对小牛胸腺DNA、聚（dA-dT）·聚（dA-dT）和聚（dA-dC）·聚（dG-dT）的构象没有显著影响。
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