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DNA序列对DNA增强铽(III)荧光的影响。

The influence of DNA sequence on terbium (III) fluorescence enhancement by DNA.

作者信息

Ringer D P, Etheredge J L, Kizer D E

出版信息

J Inorg Biochem. 1985 Jun;24(2):137-45. doi: 10.1016/0162-0134(85)80005-2.

DOI:10.1016/0162-0134(85)80005-2
PMID:4045443
Abstract

The synthetic DNA duplexes, poly(dA-dC):poly(dG-dT), poly(dG):poly(dC), poly(dG-dC):poly(dG-dC), and poly(dG-m5dC):poly(dG-m5dC), were analyzed as double- and single-strand polymers for the ability to enhance terbium fluorescence. Using conditions which limited the enhancement of Tb3+ fluorescence to that from DNA-guanosines, our results showed that (a) guanosines in single-strand DNA enhanced terbium fluorescence equally well irrespective of the primary sequence surrounding them, and (b) guanosines in either left- (Z-form) or right- (B-form) handed double helixes failed to enhance terbium fluorescence.

摘要

对合成的DNA双链体,即聚(dA-dC):聚(dG-dT)、聚(dG):聚(dC)、聚(dG-dC):聚(dG-dC)和聚(dG-m5dC):聚(dG-m5dC),作为双链和单链聚合物增强铽荧光的能力进行了分析。在将Tb3+荧光增强限制为仅源于DNA鸟苷的条件下,我们的结果表明:(a)单链DNA中的鸟苷,无论其周围的一级序列如何,增强铽荧光的效果同样良好;(b)左手(Z型)或右手(B型)双螺旋中的鸟苷均不能增强铽荧光。

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