Polyphenolic profile and antioxidant properties of L. 'Enaja' cultivar grown in Romania.

This is the first study describing phenolics of L. 'Enaja' cultivar (bitter melon) produced in Romania. Total polyphenol content, total tannin content, total flavonoid content, and antioxidant activity of bitter melon stems and leaves, young fruits, and ripe fruits grown in Romania were analysed, along with fruits imported from India. The UPLC-DAD analysis led to the identification of (+)-catechin, (-)-epicatechin, luteolin-3',7-di--glucoside, luteolin-7--glucoside and vanillic acid. (-)-Epicatechin (859 µg/g) and (+)-catechin (1677 µg/g) were the most abundant compounds in stems and leaves, while in the ripe fruits, luteolin-7--glucoside (310 µg/g) was the main phenolic. Stems and leaves were the most active for capturing free DPPH radicals (IC = 216.9 ± 11.91 µg/ml); the scavenging activity strongly correlated with the flavonoid content ( = 0.8806, r = 0.7754). fruits from Romania, both young and ripe, are a source of polyphenols as valuable as those imported from India.