Andreo-Jimenez Beatriz, Te Beest Dennis E, Kruijer Willem, Vannier Nathan, Kadam Niteen N, Melandri Giovanni, Jagadish S V Krishna, van der Linden Gerard, Ruyter-Spira Carolien, Vandenkoornhuyse Philippe, Bouwmeester Harro J
Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands.
Biointeractions and Plant Health, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
Rice (N Y). 2023 May 22;16(1):26. doi: 10.1186/s12284-023-00641-4.
Rice is the second most produced crop worldwide, but is highly susceptible to drought. Micro-organisms can potentially alleviate the effects of drought. The aim of the present study was to unravel the genetic factors involved in the rice-microbe interaction, and whether genetics play a role in rice drought tolerance. For this purpose, the composition of the root mycobiota was characterized in 296 rice accessions (Oryza sativa L. subsp. indica) under control and drought conditions. Genome wide association mapping (GWAS) resulted in the identification of ten significant (LOD > 4) single nucleotide polymorphisms (SNPs) associated with six root-associated fungi: Ceratosphaeria spp., Cladosporium spp., Boudiera spp., Chaetomium spp., and with a few fungi from the Rhizophydiales order. Four SNPs associated with fungi-mediated drought tolerance were also found. Genes located around those SNPs, such as a DEFENSIN-LIKE (DEFL) protein, EXOCYST TETHERING COMPLEX (EXO70), RAPID ALKALINIZATION FACTOR-LIKE (RALFL) protein, peroxidase and xylosyltransferase, have been shown to be involved in pathogen defense, abiotic stress responses and cell wall remodeling processes. Our study shows that rice genetics affects the recruitment of fungi, and that some fungi affect yield under drought. We identified candidate target genes for breeding to improve rice-fungal interactions and hence drought tolerance.
水稻是全球产量第二高的作物，但极易受到干旱影响。微生物有可能减轻干旱的影响。本研究的目的是揭示水稻与微生物相互作用中涉及的遗传因素，以及遗传学在水稻耐旱性中是否发挥作用。为此，在对照和干旱条件下，对296份水稻种质（亚洲栽培稻籼亚种）的根真菌群落组成进行了表征。全基因组关联图谱分析（GWAS）鉴定出与六种根相关真菌相关的十个显著（LOD>4）单核苷酸多态性（SNP）：球腔菌属、枝孢属、布迪拉属、毛壳菌属，以及一些根壶菌目的真菌。还发现了四个与真菌介导的耐旱性相关的SNP。位于这些SNP附近的基因，如类防御素（DEFL）蛋白、外泌体拴系复合体（EXO70）、类快速碱化因子（RALFL）蛋白、过氧化物酶和木糖基转移酶，已被证明参与病原体防御、非生物胁迫反应和细胞壁重塑过程。我们的研究表明，水稻遗传学影响真菌的募集，并且一些真菌在干旱条件下影响产量。我们鉴定出了用于育种的候选靶基因，以改善水稻与真菌的相互作用，从而提高耐旱性。
