Huang Yiyao, Arab Tanina, Russell Ashley E, Mallick Emily R, Nagaraj Rajini, Gizzie Evan, Redding-Ochoa Javier, Troncoso Juan C, Pletnikova Olga, Turchinovich Andrey, Routenberg David A, Witwer Kenneth W
Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Biology, School of Science, Penn State Erie, The Behrend College, Erie, PA, United States.
bioRxiv. 2023 May 13:2023.05.06.539665. doi: 10.1101/2023.05.06.539665.
Extracellular vesicles (EVs) are released from different cell types in the central nervous system (CNS) and play roles in regulating physiological and pathological functions. Although brain-derived EVs (bdEVs) have been successfully collected from brain tissue, there is not yet a "bdEV atlas" of EVs from different brain regions. To address this gap, we separated EVs from eight anatomical brain regions of a single individual and subsequently characterized them by count, size, morphology, and protein and RNA content. The greatest particle yield was from cerebellum, while the fewest particles were recovered from the orbitofrontal, postcentral gyrus, and thalamus regions. EV surface phenotyping indicated that CD81 and CD9 were more abundant than CD63 for all regions. Cell-enriched surface markers varied between brain regions. For example, putative neuronal markers NCAM, CD271, and NRCAM were more abundant in medulla, cerebellum, and occipital regions, respectively. These findings, while restricted to tissues from a single individual, suggest that additional studies are merited to lend more insight into the links between EV heterogeneity and function in the CNS.
细胞外囊泡（EVs）由中枢神经系统（CNS）中的不同细胞类型释放，并在调节生理和病理功能中发挥作用。尽管已成功从脑组织中收集到脑源性细胞外囊泡（bdEVs），但尚未有来自不同脑区的bdEVs的“图谱”。为了填补这一空白，我们从一个个体的八个脑解剖区域分离出细胞外囊泡，随后通过数量、大小、形态以及蛋白质和RNA含量对其进行表征。最大的颗粒产量来自小脑，而从眶额叶、中央后回和丘脑区域回收的颗粒最少。细胞外囊泡表面表型分析表明，所有区域中CD81和CD9比CD63更丰富。富含细胞的表面标志物在不同脑区有所不同。例如，假定的神经元标志物NCAM、CD271和NRCAM分别在延髓、小脑和枕叶区域更为丰富。这些发现虽然仅限于来自单个个体的组织，但表明值得进行更多研究，以更深入地了解细胞外囊泡异质性与中枢神经系统功能之间的联系。
