Jeon Woo-Young, Jin Seong Eun, Sohn Eunjin, Jo Kyuhyung, Ha Hyekyung, Shin Hyeun-Kyoo, Lee Mee-Young
KM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
KM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
Heliyon. 2023 May 11;9(5):e16172. doi: 10.1016/j.heliyon.2023.e16172. eCollection 2023 May.
Cheonwangbosim-dan is a traditional herbal prescription that is widely used to improve or treat physical and mental illnesses in East Asian countries.Aim of the study: The aim of the present study was to investigate the preventive and protective effects of a Cheonwangbosim-dan water extract (CBDW) against allergic inflammation using and models.
BEAS-2B and MC/9 cells were treated with various concentrations of CBDW and stimulated with different inducers of inflammatory mediators. The production of various inflammatory mediators was subsequently evaluated. BALB/c mice were sensitized and challenged by repeated application of ovalbumin (OVA). CBDW was administered by oral gavage once daily for 10 consecutive days. We assessed the number of inflammatory cells and production of Th2 cytokines in bronchoalveolar lavage fluid (BALF), the plasma levels of total and OVA-specific immunoglobulin E (IgE), and histological changes in lung tissue.
Our findings showed that CBDW significantly decreased the levels of various inflammatory mediators (eotaxin-1, eotaxin-3, RANTES, LTC, TNF-α, MMP-9, 5-LO, ICAM-1, and VCAM-1) , significantly reduced the accumulation of total inflammatory cells, the production of Th2 cytokines (IL-5 and IL-13), the levels of IgE (total and OVA-specific) , and remarkably inhibited histological changes (infiltration of inflammatory cells and goblet cell hyperplasia) .
These results suggest that CBDW possesses anti-inflammatory and anti-allergic properties by lowering allergic inflammation.
天王补心丹是一种传统草药方剂，在东亚国家被广泛用于改善或治疗身心疾病。
本研究旨在使用[具体模型1]和[具体模型2]模型，研究天王补心丹水提取物（CBDW）对过敏性炎症的预防和保护作用。
用不同浓度的CBDW处理BEAS-2B和MC/9细胞，并用不同的炎症介质诱导剂进行刺激。随后评估各种炎症介质的产生。通过反复给予卵清蛋白（OVA）使BALB/c小鼠致敏并激发。连续10天每天通过口服灌胃给予CBDW。我们评估了支气管肺泡灌洗液（BALF）中炎症细胞的数量和Th2细胞因子的产生、总免疫球蛋白E（IgE）和OVA特异性IgE的血浆水平以及肺组织的组织学变化。
我们的研究结果表明，CBDW显著降低了各种炎症介质（嗜酸性粒细胞趋化蛋白-1、嗜酸性粒细胞趋化蛋白-3、调节活化正常T细胞表达和分泌因子、白三烯C、肿瘤坏死因子-α、基质金属蛋白酶-9、5-脂氧合酶、细胞间黏附分子-1和血管细胞黏附分子-1）的水平，显著减少了总炎症细胞的积聚、Th2细胞因子（白细胞介素-5和白细胞介素-13）的产生、IgE（总IgE和OVA特异性IgE）的水平，并显著抑制了组织学变化（炎症细胞浸润和杯状细胞增生）。
这些结果表明，CBDW通过减轻过敏性炎症具有抗炎和抗过敏特性。
