Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah51452, Saudi Arabia.
Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah51452, Saudi Arabia.
Oxid Med Cell Longev. 2020 Jun 14;2020:3620192. doi: 10.1155/2020/3620192. eCollection 2020.
Asthma is characterized by the elevated level of Th2 immune responses, oxidative stress, and airway inflammation. Bilsaan, an exudate from the stem of , has been traditionally used in the treatment of various ailments in Saudi Arabia. Here, we investigated the therapeutic potential of Bilsaan against ovalbumin- (OVA-) induced allergic asthma in a mouse model. In order to induce allergic asthma, mice were intraperitoneally injected with alum-emulsified-OVA (20 g/mouse) on days 0, 14, and 21 that is followed by an intranasal OVA exposure from days 22 to 30. During this time, mice were orally administered with Bilsaan at the doses of 5, 10, and 25 mg/kg. The numbers of total and differential inflammatory cells and the levels of Th2 cytokines (IL-4, IL-5, and IL-13) and IgE were determined in bronchoalveolar lavage fluid (BALF). Moreover, the therapeutic effect of Bilsaan was also assessed to analyze the oxidative stress and inflammatory changes in the lung tissues. The results demonstrated that Bilsaan treatment significantly reduced the total and differential inflammatory cell count in the BALF. The BALF from the mice treated with Bilsaan showed significantly lower levels of IL-4, IL-5, IL-13, and IgE. Interestingly, a similar pattern was observed in IL-4, IL-5, and IL-13 secreted by OVA-sensitized splenocytes from the mice of various groups. Bilsaan treatment alleviated the status of oxidative stress by modulating malondialdehyde (MDA), superoxide dismutase (SOD), and catalase levels in the lung. Moreover, Bilsaan treatment reduced the infiltration of inflammatory cells, thickening of alveolar wall, and congestion in the lung tissues. The findings of the present study demonstrated an antiasthmatic effect of Bilsaan through the modulation of Th2 immune responses, inflammation, and the oxidative stress.
哮喘的特征是 Th2 免疫应答、氧化应激和气道炎症水平升高。Bilsaan 是 的茎渗出物，在沙特阿拉伯传统上用于治疗各种疾病。在这里，我们研究了 Bilsaan 对卵清蛋白（OVA）诱导的过敏性哮喘的治疗潜力，使用了小鼠模型。为了诱导过敏性哮喘，在第 0、14 和 21 天，用 alum 乳化的 OVA（20μg/只）对小鼠进行腹腔注射，然后在第 22 到 30 天通过鼻腔 OVA 暴露进行处理。在此期间，以 5、10 和 25mg/kg 的剂量给小鼠口服 Bilsaan。在支气管肺泡灌洗液（BALF）中测定总细胞和分类细胞的数量，以及 Th2 细胞因子（IL-4、IL-5 和 IL-13）和 IgE 的水平。此外，还评估了 Bilsaan 的治疗效果，以分析肺组织中的氧化应激和炎症变化。结果表明，Bilsaan 治疗显著减少了 BALF 中的总细胞和分类细胞计数。Bilsaan 治疗组的 BALF 中 IL-4、IL-5、IL-13 和 IgE 的水平显著降低。有趣的是，在各组小鼠的 OVA 致敏脾细胞分泌的 IL-4、IL-5 和 IL-13 中也观察到了类似的模式。Bilsaan 治疗通过调节肺中的丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶水平来缓解氧化应激状态。此外，Bilsaan 治疗减少了肺组织中炎症细胞的浸润、肺泡壁增厚和充血。本研究的结果表明，Bilsaan 通过调节 Th2 免疫应答、炎症和氧化应激来发挥抗哮喘作用。
