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LldR 对 K-12 基因组转录调控的扩展作用：乳酸利用和酸抗性

Expanded roles of lactate-sensing LldR in transcription regulation of the K-12 genome: lactate utilisation and acid resistance.

机构信息

School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.

Micro-Nano Technology Research Center, Hosei University, Koganei, Tokyo, Japan.

出版信息

Microb Genom. 2023 May;9(5). doi: 10.1099/mgen.0.001015.

DOI:10.1099/mgen.0.001015
PMID:37219924
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10272880/
Abstract

LldR is a lactate-responsive transcription factor (TF) that transcriptionally regulates the operon consisting of lactate permease and lactate dehydrogenase. The operon facilitates the utilisation of lactic acid in bacteria. However, the role of LldR in whole genomic transcriptional regulation, and the mechanism involved in adaptation to lactate remains unclear. We used genomic SELEX (gSELEX) to comprehensively analyse the genomic regulatory network of LldR to understand the overall regulatory mechanism of lactic acid adaptation of the model intestinal bacterium . In addition to the involvement of the operon in utilising lactate as a carbon source, genes related to glutamate-dependent acid resistance and altering the composition of membrane lipids were identified as novel targets of LldR. A series of and regulatory analyses led to the identification of LldR as an activator of these genes. Furthermore, the results of lactic acid tolerance tests and co-culture experiments with lactic acid bacteria suggested that LldR plays a significant role in adapting to the acid stress induced by lactic acid. Therefore, we propose that LldR is an l-/d-lactate sensing TF for utilising lactate as a carbon source and for resistance to lactate-induced acid stress in intestinal bacteria.

摘要

LldR 是一种乳酸响应转录因子 (TF)，它转录调节由乳酸透性酶和乳酸脱氢酶组成的 操纵子。该操纵子有助于细菌利用乳酸。然而，LldR 在全基因组转录调控中的作用以及适应乳酸的相关机制仍不清楚。我们使用基因组 SELEX (gSELEX) 全面分析了 LldR 的基因组调控网络，以了解模型肠道细菌乳酸适应的整体调控机制。除了 操纵子参与利用乳酸作为碳源外，还确定了与谷氨酸依赖的酸抗性和改变膜脂组成相关的基因是 LldR 的新靶标。一系列 和 调控分析表明，LldR 是这些基因的激活剂。此外，乳酸耐受试验和与乳酸菌共培养实验的结果表明，LldR 在适应由乳酸引起的酸应激中发挥重要作用。因此，我们提出 LldR 是一种 l-/d-乳酸感应 TF，用于利用乳酸作为碳源以及抵抗肠道细菌中由乳酸诱导的酸应激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/1cfe404a7ceb/mgen-9-1015-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/d0bcb8e90a9b/mgen-9-1015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/245a814884ff/mgen-9-1015-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/b7715da62002/mgen-9-1015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/7e94cb66247e/mgen-9-1015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/4f20e85a5bd6/mgen-9-1015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/9a9a6ee66d8e/mgen-9-1015-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/7a6b79a7b316/mgen-9-1015-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/766cee8f8029/mgen-9-1015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/e9becff67cf1/mgen-9-1015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/1cfe404a7ceb/mgen-9-1015-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/d0bcb8e90a9b/mgen-9-1015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/245a814884ff/mgen-9-1015-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/b7715da62002/mgen-9-1015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/7e94cb66247e/mgen-9-1015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/4f20e85a5bd6/mgen-9-1015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/9a9a6ee66d8e/mgen-9-1015-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/7a6b79a7b316/mgen-9-1015-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/766cee8f8029/mgen-9-1015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/e9becff67cf1/mgen-9-1015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547f/10272880/1cfe404a7ceb/mgen-9-1015-g0010.jpg

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