Diolez A, Richaud F, Coleno A
J Bacteriol. 1986 Jul;167(1):400-3. doi: 10.1128/jb.167.1.400-403.1986.
The pelB gene, which encodes one of the five pectate lyase isoenzymes of Erwinia chrysanthemi 3937, was mutagenized with a mini-Mu transposable element that can form gene fusions to the neomycin phosphotransferase-encoding region. Secondary mutants resistant to kanamycin in the absence of polygalacturonate, an inducer of wild-type pectate lyase activities, were selected. Such mutants produced other pectate lyase isoenzymes in the absence of the inducer.
pelB基因编码菊欧文氏菌3937的五种果胶酸裂解酶同工酶之一，用一种能与编码新霉素磷酸转移酶的区域形成基因融合的微型Mu转座元件对其进行诱变。在没有野生型果胶酸裂解酶活性诱导剂聚半乳糖醛酸的情况下，筛选出对卡那霉素有抗性的二级突变体。这类突变体在没有诱导剂的情况下能产生其他果胶酸裂解酶同工酶。
