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在体克隆菊欧文氏菌果胶酶和纤维素酶基因。

In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.

机构信息

Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.

出版信息

EMBO J. 1985 Mar;4(3):787-92. doi: 10.1002/j.1460-2075.1985.tb03698.x.

DOI:10.1002/j.1460-2075.1985.tb03698.x
PMID:16453607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554257/
Abstract

Using an RP4 plasmid which carries a mini-Mu prophage which allows it to integrate spontaneously random pieces of its host chromosome, we cloned in vivo at least some of the pectate lyase and cellulase genes of the Erwinia chrysanthemi strain B374. The RP4-prime plasmids were used to localize the cloned genes on the B374 chromosome by co-transposition mapping and to subclone most of the genes in a classic high copy number plasmid vector.

摘要

我们利用携带 mini-Mu 噬菌体的 RP4 质粒，使它能够随机整合宿主染色体的随机片段，从菊欧文氏菌 B374 菌株中克隆了至少一些果胶裂解酶和纤维素酶基因。我们使用 RP4-prime 质粒通过共转座作图将克隆基因定位到 B374 染色体上，并将大部分基因亚克隆到经典的高拷贝数质粒载体中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/393785aa2c77/emboj00268-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/aad9b9f267eb/emboj00268-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/476761dd1444/emboj00268-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/393785aa2c77/emboj00268-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/aad9b9f267eb/emboj00268-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/476761dd1444/emboj00268-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/467c/554257/393785aa2c77/emboj00268-0209-a.jpg

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In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.在体克隆菊欧文氏菌果胶酶和纤维素酶基因。
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Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi.欧文氏菌果胶酸裂解酶基因中的Mu - lac插入定向诱变
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引用本文的文献

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2
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Appl Environ Microbiol. 1987 Oct;53(10):2315-20. doi: 10.1128/aem.53.10.2315-2320.1987.
3

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TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12.大肠杆菌K-12中可共转导精氨酸突变的拓扑学
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[The reciprocal antagonistic properties among the Erwinia. Discussion of the taxonomic position of this genus].[欧文氏菌属之间的相互拮抗特性。该属分类地位的讨论]
PehN, a polygalacturonase homologue with a low hydrolase activity, is coregulated with the other Erwinia chrysanthemi polygalacturonases.
PehN是一种具有低水解酶活性的多聚半乳糖醛酸酶同系物，与其他菊欧文氏菌多聚半乳糖醛酸酶共同调控。
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Resolution of four pectate lyase structural genes of Erwinia chrysanthemi (EC16) and characterization of the enzymes produced in Escherichia coli.菊欧文氏菌（EC16）四个果胶酸裂解酶结构基因的解析及在大肠杆菌中产生的酶的特性分析。
Mol Gen Genet. 1987 Sep;209(2):319-25. doi: 10.1007/BF00329660.
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Osmoregulated periplasmic glucan synthesis is required for Erwinia chrysanthemi pathogenicity.渗透压调节的周质葡聚糖合成是菊欧文氏菌致病性所必需的。
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Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937.菊欧文氏菌3937的胞外多聚半乳糖醛酸裂解酶PelX的特性分析
J Bacteriol. 1999 Mar;181(5):1652-63. doi: 10.1128/JB.181.5.1652-1663.1999.
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Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.菊欧文氏菌3937的果胶酸裂解酶PelI属于一个新家族。
J Bacteriol. 1997 Dec;179(23):7321-30. doi: 10.1128/jb.179.23.7321-7330.1997.
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Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.菊欧文氏菌果胶酸裂解酶基因调控突变体
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Annu Rev Microbiol. 1980;34:645-76. doi: 10.1146/annurev.mi.34.100180.003241.
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Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.在电泳或电聚焦后，或在滴定曲线中检测解聚酶同工酶。
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Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.菊花欧文氏菌中果胶酶和纤维素酶分泌缺陷的突变体。
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Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
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