Department of Pediatric Surgery, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Türkiye.
Department of Histology-Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Türkiye.
Pediatr Surg Int. 2023 May 24;39(1):204. doi: 10.1007/s00383-023-05487-x.
To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia-reperfusion (I/R) injury.
AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exosomes from AD-MSCs were obtained with the miRCURY exosome isolation kit. 21 rats were divided into 3 groups. The I/R model was created as 720° torsion for 4 h and reperfusion for 4 h. In the Sham group (SG), only scrotal incision was made. 100 µl of medium in the torsion-control group (T-CG) and 100 µl of exosome in the treatment group (TG) were injected into the testicular parenchyma after detorsion. Johnsen scores of testicles were determined. Apoptosis was evaluated by the TUNEL method.
It was observed that the seminiferous tubule structures were partially disrupted in T-CG, but normal in SG and TG. Johnsen scores in SG, T-CG, and TG were 8.64 ± 0.39, 7.71 ± 0.37, and 8.57 ± 0.39, respectively. Apoptotic cell distribution was 11.28 ± 5.25%, 60.58% ± 1.68% and 17.71 ± 8.34% in SG, T-CG and TG, respectively. In both parameters, the difference between SG and TG was insignificant (p > 0.05), the difference between T-CG/TG and SG/T-CG was significant (p < 0.05).
Exosomes obtained from AD-MSCs are effective in preventing testicular I/R injury. This effect appears to occur because of suppression of apoptotic activity.
研究脂肪间充质干细胞（AD-MSCs）来源的外泌体对睾丸缺血再灌注（I/R）损伤的影响。
培养大鼠脂肪组织来源的 AD-MSCs。通过 CD44、CD90、CD34 和 CD45 抗体评估细胞的特征。使用 miRCURY 外泌体分离试剂盒获得 AD-MSCs 来源的外泌体。将 21 只大鼠分为 3 组。I/R 模型为 4 h 旋转 720°并再灌注 4 h。在 Sham 组（SG）中，仅进行阴囊切开。在扭转对照组（T-CG）中注射 100 µl 培养基，在治疗组（TG）中注射 100 µl 外泌体，在扭转后注入睾丸实质。测定睾丸的 Johnsen 评分。通过 TUNEL 法评估细胞凋亡。
T-CG 中部分观察到曲细精管结构破坏，但 SG 和 TG 中正常。SG、T-CG 和 TG 的 Johnsen 评分分别为 8.64±0.39、7.71±0.37 和 8.57±0.39。SG、T-CG 和 TG 中的凋亡细胞分布分别为 11.28±5.25%、60.58%±1.68%和 17.71±8.34%。在这两个参数中，SG 和 TG 之间的差异无统计学意义（p>0.05），T-CG/TG 和 SG/T-CG 之间的差异有统计学意义（p<0.05）。
AD-MSCs 来源的外泌体可有效预防睾丸 I/R 损伤。这种作用似乎是通过抑制细胞凋亡活性来实现的。
