Applied Environmental and Geomicrobiology Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Zoology, Rawalpindi Women University, Rawalpindi, Pakistan.
Biotechnol Lett. 2023 Aug;45(8):967-979. doi: 10.1007/s10529-023-03390-y. Epub 2023 May 25.
At currently, approximately 70 species of magnetotactic bacteria have been identified; thus, there is an urgent need to identify more magnetotactic bacteria from diverse environmental sources with potential applications in industry and biotechnology. To the best of our knowledge, this is the first magnetotactic bacterial strain discovered in Pakistan. The first magnetotactic bacteria, Magnetospirillum moscoviense MS-24, was isolated from Banjosa Lake (Rawalakot), Pakistan, in the current investigation. Magnetospirillum moscoviense MS-24 was screened using the Racetrack method. The Magnetospirillum moscoviense MS-24 were physically characterised using Atomic Force Microscopy, High-Resolution Scanning Electron Microscopy, and Transmission Electron Microscopy. The current study used microscopy to illustrate the shape of bacteria and to find a very obvious chain of magnetosomes within the bacterial cell. The Magnetospirillum moscoviense MS-24 measured about 4 ± 0.04 µm in length and 600 ± 0.02 nm in diameter. The microfluidic chip experiments were also used to detect magnetotaxis behaviour in bacteria.
目前已鉴定出约 70 种磁细菌；因此，迫切需要从不同的环境来源中鉴定出更多具有工业和生物技术应用潜力的磁细菌。据我们所知，这是在巴基斯坦发现的第一株磁细菌菌株。在本研究中，首次从巴基斯坦拉瓦尔品第的班若萨湖（Banjosa Lake）中分离出了趋磁细菌 Magnetospirillum moscoviense MS-24。使用轨迹法筛选出 Magnetospirillum moscoviense MS-24。使用原子力显微镜、高分辨率扫描电子显微镜和透射电子显微镜对 Magnetospirillum moscoviense MS-24 进行了物理特性分析。本研究使用显微镜来描述细菌的形状，并在细菌细胞内发现了一条非常明显的磁小体链。Magnetospirillum moscoviense MS-24 的长度约为 4 ± 0.04 μm，直径约为 600 ± 0.02 nm。还使用微流控芯片实验来检测细菌的趋磁行为。
